A new tool for probing of cell–cell communication: human embryonic germ cells inducing apoptosis of SKOV3 ovarian cancer cells on a microfluidic chip
A new tool for probing of cell–cell communication: human embryonic germ cells inducing apoptosis of SKOV3 ovarian cancer cells on a microfluidic chip
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探测细胞间通讯的新工具：人胚胎生殖细胞在微流控芯片上诱导 SKOV3 卵巢癌细胞凋亡
DOI：
10.1007/s10529-008-9725-2
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发表时间：
2008
影响因子：
2.7
通讯作者：
Dong Li
中科院分区：
文献类型：
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作者：
Xinyan Song;B. Kong;Dong Li
建立了一种单向灌流的微流控装置，用于观察人胚胎生殖细胞（hEG）对SKOV3细胞的作用。将hEG和SK0V3细胞分别接种在入口和出口储器中，并共培养2天。培养基从入口到出口单向灌注。在线监测SKOV3细胞生长抑制情况，SKOV3培养区凋亡信号沿着培养基流动而减弱。总之，微流控芯片是一个潜在的有用的工具，研究干细胞对癌细胞的作用与直观的细胞为基础的筛选。
A microfluidic device with unidirectional perfusion has been developed to observe the effect of human embryonic germ (hEG) cells on SKOV3 cells. The hEG and SKOV3 cells were seeded in the inlet and the outlet reservoirs separately, and co-cultured for 2 days. The medium was perfused unidirectionally from the inlet to the outlet. The growth inhibition of SKOV3 cells was monitored online and the apoptosis signals in SKOV3 culture area decreased along the flow of the medium. In conclusion, microfluidic chip is a potentially useful tool to investigate the effect of stem cells on cancer cells with intuitionistic cell-based screens.
DOI：
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发表时间：
2012-05
期刊：
--
影响因子：
--
作者：
Ke Wu
通讯作者：
Ke Wu
影响因子：
14
作者：
Nie, Fu-Qiang;Yamada, Masumi;Okano, Teruo
通讯作者：
Okano, Teruo