Circulating eNAMPT as a biomarker in the critically ill: acute pancreatitis, sepsis, trauma, and acute respiratory distress syndrome.
Circulating eNAMPT as a biomarker in the critically ill: acute pancreatitis, sepsis, trauma, and acute respiratory distress syndrome.
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DOI：
10.1186/s12871-022-01718-1
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发表时间：
2022-06-15
影响因子：
2.2
通讯作者：
Garcia, Joe G. N.
中科院分区：
文献类型：
--
作者：
Bime, Christian;Casanova, Nancy G.;Camp, Sara M.;Oita, Radu C.;Ndukum, Juliet;Hernon, Vivian Reyes;Oh, Dong Kyu;Li, Yansong;Greer, Phil J.;Whitcomb, David C.;Papachristou, Georgios, I;Garcia, Joe G. N.
烟酰胺磷酸核糖基转移酶(NAMPT)具有双重功能，一种是细胞内调节烟酰胺腺嘌呤二核苷酸代谢的酶，另一种是细胞外分泌的蛋白，通过与Toll样受体4结合和核因子κB的激活，发挥天然免疫的细胞因子调节作用。在有限的临床前和临床研究中，eNAMPT与急性呼吸窘迫综合征(ARDS)的病理生物学有关，提示eNAMPT可能作为诊断和预后的生物标志物。我们研究了循环eNAMPT水平作为ARDS和ARDS易感疾病患者扩大队列中生物标记物的可行性，这些患者包括急性胰腺炎、脓毒症和创伤，并与对照组进行了比较。共有671名患者和179名健康对照被纳入两个独立的队列。血浆和血清eNAMPT水平采用两种互补的酶联免疫吸附分析法中的一种进行定量。在血浆/血清eNAMPT测量的对数基数2方差稳定化转换后，在适用的情况下，使用线性回归或广义估计方程(GEE)模型比较健康对照组和每个疾病队列之间的差异。补充分析包括敏感度、特异度、阳性预测值、阴性预测值和受试者工作曲线下面积。与对照组相比，急性胰腺炎、脓毒症、创伤和急性呼吸窘迫综合征患者的循环eNAMPT水平显著升高(均p <； 0.01)。在急性胰腺炎队列中，循环eNAMPT水平与疾病严重程度呈正相关(p <； 0.01)。循环eNAMPT水平是急性胰腺炎、脓毒症、创伤和/或ARDS危重患者的新生物标志物，有可能反映疾病的严重程度。网上版载有补充材料，可在10.1186/s12871-022-01718-1查阅。
Nicotinamide phosphoribosyltransferase (NAMPT) exhibits dual functionality – as an intracellular enzyme regulating nicotinamide adenine dinucleotide metabolism and as an extracellular secreted protein (eNAMPT) to function as a cytokine regulator of innate immunity via binding to Toll-Like receptor 4 and NF-κB activation. In limited preclinical and clinical studies, eNAMPT was implicated in the pathobiology of acute respiratory distress syndrome (ARDS) suggesting that eNAMPT could potentially serve as a diagnostic and prognostic biomarker. We investigated the feasibility of circulating eNAMPT levels to serve as a biomarker in an expanded cohort of patients with ARDS and ARDS-predisposing conditions that included acute pancreatitis, sepsis, and trauma with comparisons to controls. A total of 671 patients and 179 healthy controls were included in two independent cohorts. Plasma and serum eNAMPT levels were quantified using one of two complementary Enzyme-linked Immunosorbent Assays. After log base 2 variance stabilizing transformation of plasma/serum eNAMPT measurements, differences between healthy controls and each disease cohort were compared using linear regression or a generalized estimating equation (GEE) model where applicable. Complementary analyses included sensitivity, specificity, positive predictive values, negative predictive values, and the area under the receiver operating curve. Compared to controls, circulating eNAMPT levels were significantly elevated in subjects with acute pancreatitis, sepsis, trauma, and ARDS (all p < 0.01). In the acute pancreatitis cohort, circulating eNAMPT levels positively correlated with disease severity (p < 0.01). Circulating eNAMPT levels are novel biomarker in the critically ill with acute pancreatitis, sepsis, trauma, and/or ARDS with the potential to reflect disease severity. The online version contains supplementary material available at 10.1186/s12871-022-01718-1.
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影响因子：
11.1
作者：
Sun BL;Sun X;Casanova N;Garcia AN;Oita R;Algotar AM;Camp SM;Hernon VR;Gregory T;Cress AE;Garcia JGN
通讯作者：
Garcia JGN
DOI：
10.1164/rccm.201208-1460oc
发表时间：
2013-04-01
影响因子：
24.7
作者：
Agrawal, Ashish;Matthay, Michael A.;Calfee, Carolyn S.
通讯作者：
Calfee, Carolyn S.
影响因子：
6.6
作者：
Lynn, Heather;Sun, Xiaoguang;Garcia, Joe G. N.
通讯作者：
Garcia, Joe G. N.
DOI：
10.1165/rcmb.2014-0117oc
发表时间：
2014-11-01
影响因子：
6.4
作者：
Sun, Xiaoguang;Elangovan, Venkateswaran Ramamoorthi;Garcia, Joe G. N.
通讯作者：
Garcia, Joe G. N.
急性呼吸窘迫综合征生物标志物的病理生理方法。
影响因子：
--
作者：
Blondonnet R;Constantin JM;Sapin V;Jabaudon M
通讯作者：
Jabaudon M