CD8 T cell response and evolutionary pressure to HIV-1 cryptic epitopes derived from antisense transcription.
CD8 T cell response and evolutionary pressure to HIV-1 cryptic epitopes derived from antisense transcription.
复制标题
DOI：
10.1084/jem.20092060
复制
发表时间：
2010-01-18
期刊：
影响因子：
--
通讯作者：
Goepfert PA
中科院分区：
文献类型：
--
作者：
Bansal A;Carlson J;Yan J;Akinsiku OT;Schaefer M;Sabbaj S;Bet A;Levy DN;Heath S;Tang J;Kaslow RA;Walker BD;Ndung'u T;Goulder PJ;Heckerman D;Hunter E;Goepfert PA
Retroviruses pack multiple genes into relatively small genomes by encoding several genes in the same genomic region with overlapping reading frames. Both sense and antisense HIV-1 transcripts contain open reading frames for known functional proteins as well as numerous alternative reading frames (ARFs). At least some ARFs have the potential to encode proteins of unknown function, and their antigenic properties can be considered as cryptic epitopes (CEs). To examine the extent of active immune response to virally encoded CEs, we analyzed human leukocyte antigen class I–associated polymorphisms in HIV-1 gag, pol, and nef genes from a large cohort of South Africans with chronic infection. In all, 391 CEs and 168 conventional epitopes were predicted, with the majority (307; 79%) of CEs derived from antisense transcripts. In further evaluation of CD8 T cell responses to a subset of the predicted CEs in patients with primary or chronic infection, both sense- and antisense-encoded CEs were immunogenic at both stages of infection. In addition, CEs often mutated during the first year of infection, which was consistent with immune selection for escape variants. These findings indicate that the HIV-1 genome might encode and deploy a large potential repertoire of unconventional epitopes to enhance vaccine-induced antiviral immunity.
登录
查看更多内容
DOI：
10.1084/jem.186.7.1051
发表时间：
1997-10-06
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Bullock TN;Patterson AE;Franlin LL;Notidis E;Eisenlohr LC
通讯作者：
Eisenlohr LC
影响因子：
3.3
作者：
Landry S;Halin M;Lefort S;Audet B;Vaquero C;Mesnard JM;Barbeau B
通讯作者：
Barbeau B
影响因子：
5.4
作者：
Masemola, A;Mashishi, T;Gray, CM
通讯作者：
Gray, CM
影响因子：
5.4
作者：
Bansal, Anju;Jackson, Bethany;Goepfert, Paul A.
通讯作者：
Goepfert, Paul A.
影响因子：
--
作者：
Garrison, Keith E.;Champiat, Stephane;Nixon, Douglas F.
通讯作者：
Nixon, Douglas F.