Evaluation of the effect of maternally derived antibody on response to MMR vaccine in Thai infants.
Evaluation of the effect of maternally derived antibody on response to MMR vaccine in Thai infants.
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DOI：
10.1016/j.vaccine.2022.01.049
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发表时间：
2022-03-01
期刊：
影响因子：
5.5
通讯作者：
Hosie MJ
中科院分区：
文献类型：
--
作者：
Hu S;Logan N;Puenpa J;Wanlapakorn N;Vongpunsawad S;Poovorawan Y;Willett BJ;Hosie MJ
尽管全球范围内的麻疹病例数量随着抗麻疹免疫运动的开展而下降，但麻疹又重新出现了。需要审查现行的疫苗接种政策，以改进消除麻疹的战略。采用伪型病毒中和试验(PVNA)检测了泰国婴儿在接种两剂MMR(1和2)疫苗前和接种后6个时间点的血清中和抗体滴度(ClinicalTrials.gov no.NCT02408926)。制备了携带麻疹病毒(MeV)血凝素(H)和融合(F)糖蛋白的水疱性口炎病毒(VSV)荧光素酶假型。将一系列稀释度的血清样本与VSV(MeV)伪型孵育，并将其接种到HEK293-人SLAM1细胞上；中和抗体滴度被定义为导致荧光素酶活性下降90%的稀释度。在第一次接种MMR疫苗时，具有高母体免疫力的出生婴儿(H组)的中和抗体滴度持续存在，这些婴儿没有通过产生保护性滴度来有效反应。相反，母亲免疫力较低的婴儿(L组)在接种疫苗后产生了保护性抗体滴度。H组对第二次MMR疫苗的应答率显著高于H组(P<0.0171)。在泰国婴儿中观察到的抗-MeV免疫球蛋白水平和中和抗体滴度之间的相关性表明，有可能使用快速免疫球蛋白检测作为中和活性的替代措施，以确定人群中的临床保护水平。这些结果表明，根据母体获得性免疫水平改变第一次MMR免疫接种的时间可以提高疫苗的免疫原性，从而加速消除麻疹。
Although the number of measles cases declined globally in response to anti-measles immunisation campaigns, measles has re-emerged. A review of current vaccination policies is required to improve measles elimination strategies. A pseudotype-based virus neutralisation assay (PVNA) was used to measure neutralising antibody titres in serum samples collected from Thai infants at six timepoints before and after two-doses of MMR (1&2) vaccination (ClinicalTrials.gov no. NCT02408926). Vesicular stomatitis virus (VSV) luciferase pseudotypes bearing the haemaglutinin (H) and fusion (F) glycoproteins of measles virus (MeV) were prepared. Serial dilutions of serum samples were incubated with VSV (MeV) pseudotypes and plated onto HEK293-human SLAM1 cells; the neutralising antibody titre was defined as the dilution resulting in 90% reduction in luciferase activity. Neutralising antibody titres in infants born with high levels of maternal immunity (H group) persisted at the time of the first MMR vaccination, and those infants did not respond effectively by developing protective titres. In contrast, infants with lower maternal immunity (L group) developed protective titres of antibody following vaccination. Responses to the second MMR vaccination were significantly higher (P = 0.0171, Wilcoxon signed-rank test) in the H group. The observed correlation between anti-MeV IgG level and neutralising antibody titre in Thai infants indicates the possibility of using rapid IgG testing as a surrogate measure for neutralising activity to define clinical protection levels within populations. These results demonstrate that varying the timing of the first MMR immunisation according to the level of acquired maternal immunity could increase vaccination immunogenicity and hence accelerate measles eradication.
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影响因子：
19
作者：
Filia, A.;Amendola, A.;Magurano, F.
通讯作者：
Magurano, F.
影响因子：
5.5
作者：
Logan N;McMonagle E;Drew AA;Takahashi E;McDonald M;Baron MD;Gilbert M;Cleaveland S;Haydon DT;Hosie MJ;Willett BJ
通讯作者：
Willett BJ
影响因子：
5.1
作者：
ALBRECHT, P;ENNIS, FA;KRUGMAN, S
通讯作者：
KRUGMAN, S
影响因子：
5.5
作者：
Mossong, J;Muller, CP
通讯作者：
Muller, CP
影响因子：
5.3
作者：
DUBRIDGE, RB;TANG, P;CALOS, MP
通讯作者：
CALOS, MP