Cloning and expression of a transcription factor activator protein-1 member identified from the swimming crab Portunus trituberculatus
Cloning and expression of a transcription factor activator protein-1 member identified from the swimming crab Portunus trituberculatus
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三疣梭子蟹转录因子激活蛋白1成员的克隆与表达
DOI：
10.1007/s12192-018-0935-9
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发表时间：
2018-09
影响因子：
3.8
通讯作者：
王春琳
中科院分区：
文献类型：
--
作者：
王欢;史策;孔梦瑶;母昌考;韦红玲;王春琳
转录激活蛋白是与靶基因的启动子区结合的调节蛋白。转录激活蛋白-1（AP-1）调节与免疫系统、细胞凋亡和增殖相关的许多基因。本研究采用表达序列标签分析和cDNA末端快速扩增技术，对三疣梭子蟹（Portunus trituberculatus，PtAP-1）AP-1基因的全长cDNA进行了鉴定。该基因全长1183 bp，编码256个氨基酸，预测分子量为28.96 kDa，等电点为8.90。PtAP-1在低盐度刺激（10%）的前6 h，在性腺组织中表达量最高，在血液、血细胞、肌肉、肝胰腺和鳃中表达量最低。此外，我们观察到鳃中PtAP-1 mRNA表达的稳定下降，但在12 h时，表达最初上调，随后显著下降，直至48 h恢复至基线水平。此外，溶藻弧菌攻击导致PtAP-1表达在前6 h显著上调，并在48 h内维持在高水平。从48到72小时，我们观察到PtAP-1水平下降，尽管它们仍然显著高于基线时检测到的水平。这些结果表明PtAP-1参与了甲壳动物的免疫应答和免疫调节。
Transcription activator proteins are regulatory proteins that bind to the promoter regions of target genes. Transcription activator protein-1 (AP-1) regulates numerous genes related to the immune system, apoptosis, and proliferation. In this study, the full-length cDNA of AP-1 from Portunus trituberculatus (PtAP-1) was identified by expressed sequence tag analysis and cDNA-end rapid amplification. The gene is 1183 bp and encodes a 256-amino acid protein with a predicted molecular mass and isoelectric point of 28.96 kDa and 8.90, respectively. PtAP-1 showed the highest expression level in the gonad tissue and the lowest expression level in blood, hemocyte, muscle, hepatopancreas, and gill, during the first 6 h of low-salinity stimulation (10%). Additionally, we observed steady decreases in PtAP-1 mRNA expression in the gill, but at 12 h, expression was initially upregulated, followed by a significant decrease until restoration to baseline levels at 48 h. Additionally, Vibrio alginolyticus challenge resulted in significant upregulation of PtAP-1 expression in the first 6 h, which was maintained at high levels for 48 h. From 48 to 72 h, we observed decreases in PtAP-1 levels, although they remained significantly higher than those detected at baseline. These results suggested that PtAP-1 is involved in the immune response and osmoregulation of crustaceans.
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影响因子：
5.3
作者：
C. Abate;D. Luk;T. Curran
通讯作者：
C. Abate;D. Luk;T. Curran
DOI：
10.1523/jneurosci.3360-15.2016
发表时间：
2016-01
期刊：
The Journal of Neuroscience
影响因子：
--
作者：
Jürgen Tuvikene;P. Pruunsild;E. Orav;Eli-Eelika Esvald;T. Timmusk
通讯作者：
Jürgen Tuvikene;P. Pruunsild;E. Orav;Eli-Eelika Esvald;T. Timmusk
影响因子：
--
作者：
W. Pearson
通讯作者：
W. Pearson
影响因子：
4
作者：
Jiongpeng Zhao;Bo-wen Hu;Qian Yang;Xiao-Feng Zhang;Tong‐Liang Hu;X. Bu
通讯作者：
Jiongpeng Zhao;Bo-wen Hu;Qian Yang;Xiao-Feng Zhang;Tong‐Liang Hu;X. Bu
影响因子：
4.7
作者：
M. De Zoysa;C. Nikapitiya;Youngdeuk Lee;Sukkyoung Lee;Chulhong Oh;I. Whang;Sang-Yeop Yeo;C. Choi;Jehee Lee
通讯作者：
M. De Zoysa;C. Nikapitiya;Youngdeuk Lee;Sukkyoung Lee;Chulhong Oh;I. Whang;Sang-Yeop Yeo;C. Choi;Jehee Lee