Imaging mass cytometry reveals the prominent role of myeloid cells at the maternal-fetal interface.
Imaging mass cytometry reveals the prominent role of myeloid cells at the maternal-fetal interface.
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成像质量细胞仪揭示了髓样细胞在母体界面界面的重要作用。
DOI：
10.1016/j.isci.2022.104648
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发表时间：
2022-07-15
期刊：
影响因子：
5.8
通讯作者：
Eikmans, Michael
中科院分区：
文献类型：
--
作者：
Krop, Juliette;van der Zwan, Anita;Ijsselsteijn, Marieke E.;Kapsenberg, Hanneke;Luk, Sietse J.;Hendriks, Sanne H.;van der Keur, Carin;Verleng, Lotte J.;Somarakis, Antonis;van der Meeren, Lotte;Haasnoot, Geert;Bos, Manon;de Miranda, Noel F. C. C.;Lopes, Susana M. Chuva de Sousa;van der Hoorn, Marie-Louise P.;Koning, Frits;Claas, Frans H. J.;Heidt, Sebastiaan;Eikmans, Michael
尽管母体-胎儿界面的免疫复杂性已得到充分认识，但母体免疫细胞和胎儿滋养层的实际相互作用尚不清楚。为了了解母体免疫细胞和胎儿绒毛外滋养细胞的组成和空间取向，我们对健康妊娠三个月的基底蜕膜应用成像质量细胞术。在所有三个月内，我们观察到蜕膜中骨髓细胞的频率比单细胞悬浮技术所观察到的要高得多。此外，它们是其他蜕膜细胞微环境中最明显的细胞类型。在妊娠早期，HLA-DR-巨噬细胞代表最丰富的骨髓亚群，并且这些细胞经常在滋养层附近观察到。足月时，HLA-DR+ 巨噬细胞亚簇大量存在，并且经常在 T 细胞的微环境中观察到。总而言之，我们的结果强调了骨髓细胞在整个妊娠期间在人类母胎界面的动态作用。组织消化后，骨髓细胞的频率被低估 骨髓细胞可以通过适当的滋养层侵袭来支持 NK 细胞 骨髓细胞在整个妊娠过程中的作用是动态的免疫学；生物技术；生物科学研究方法论
Although the immunological complexity of the maternal-fetal interface is well appreciated, the actual interaction of maternal immune cells and fetal trophoblasts is insufficiently understood. To comprehend the composition and spatial orientation of maternal immune cells and fetal extravillous trophoblasts, we applied imaging mass cytometry on decidua basalis of the three trimesters of healthy pregnancy. Within all trimesters, we observed considerably higher frequencies of myeloid cells in the decidua than is seen with single-cell suspension techniques. Moreover, they were the most pronounced cell type in the microenvironment of other decidual cells. In first trimester, HLA-DR- macrophages represented the most abundant myeloid subcluster and these cells were frequently observed in the vicinity of trophoblasts. At term, HLA-DR+ macrophage subclusters were abundantly present and frequently observed in the microenvironment of T cells. Taken together, our results highlight the dynamic role of myeloid cells at the human maternal-fetal interface throughout gestation. Frequency of myeloid cells is underestimated after tissue digestion Myeloid cells could support NK cells with proper trophoblast invasion Myeloid cells are dynamic in their role throughout gestation Biological sciences; Immunology; Biotechnology; Biological sciences research methodologies
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DOI：
10.1002/cyto.a.24480
发表时间：
2021-12
期刊：
Cytometry. Part A : the journal of the International Society for Analytical Cytology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.4
作者：
HALLER, H;RADILLO, O;RANDIC, L
通讯作者：
RANDIC, L
DOI：
10.1016/s0167-4889(99)00171-8
发表时间：
2000-02-28
影响因子：
5.1
作者：
Pfau, JC;Walker, E;Card, GL
通讯作者：
Card, GL
影响因子：
3.7
作者：
Mei, Henrik E.;Leipold, Michael D.;Maecker, Holden T.
通讯作者：
Maecker, Holden T.
影响因子：
20.3
作者：
Perez, SA;Sotiropoulou, PA;Papamichail, M
通讯作者：
Papamichail, M