Direct Determination of the Mutation Rate in the Bumblebee Reveals Evidence for Weak Recombination-Associated Mutation and an Approximate Rate Constancy in Insects.
Direct Determination of the Mutation Rate in the Bumblebee Reveals Evidence for Weak Recombination-Associated Mutation and an Approximate Rate Constancy in Insects.
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直接测定大黄蜂的突变率揭示了昆虫中弱重组相关突变和近似速率恒定性的证据
DOI：
10.1093/molbev/msw226
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发表时间：
2017-01
影响因子：
10.7
通讯作者：
Yang S
中科院分区：
文献类型：
--
作者：
Liu H;Jia Y;Sun X;Tian D;Hurst LD;Yang S
突变率的准确知识提供了一个基线，用于推断预期的进化速率，用于测试进化假设和估计关键参数。测序技术的进步现在允许从近亲的测序中直接估计突变率。在昆虫中，有三个这样的估计，两个在非社会性昆虫中（果蝇：每代每一个单倍体基因组每bp 2.8 × 10−9; Heliconius：2.9 × 10−9），一个在社会性物种蜜蜂中（3.4 × 10−9）。蜜蜂的突变率会高出20%吗？因为它有非常高的重组率，而重组可能是直接或间接的诱变性。为了解决这种可能性，我们提供了一个直接估计的突变率在熊蜂（熊蜂terrestris），这是一个近亲的蜜蜂，但重组率低得多。我们证实，大黄蜂的交叉率确实比蜜蜂低得多（8.7 cM/Mb对37 cM/Mb）。重要的是，我们发现突变率没有显著差异：我们估计大黄蜂每一代每个单倍体基因组的突变率为3.6 × 10−9（95%置信区间为2.38 × 10−9和5.37 × 10−9），仅比蜜蜂的估计高5%。两个基因组每一代每个单倍体基因组大约有一个新的突变。虽然我们发现了重组和突变之间直接耦合的证据（在蜜蜂中也可见），但这种影响非常微弱，几乎没有留下任何物种间差异的痕迹。突变率的相似性表明昆虫的突变率近似恒定。
Accurate knowledge of the mutation rate provides a base line for inferring expected rates of evolution, for testing evolutionary hypotheses and for estimation of key parameters. Advances in sequencing technology now permit direct estimates of the mutation rate from sequencing of close relatives. Within insects there have been three prior such estimates, two in nonsocial insects (Drosophila: 2.8 × 10−9 per bp per haploid genome per generation; Heliconius: 2.9 × 10−9) and one in a social species, the honeybee (3.4 × 10−9). Might the honeybee’s rate be ∼20% higher because it has an exceptionally high recombination rate and recombination may be directly or indirectly mutagenic? To address this possibility, we provide a direct estimate of the mutation rate in the bumblebee (Bombus terrestris), this being a close relative of the honeybee but with a much lower recombination rate. We confirm that the crossover rate of the bumblebee is indeed much lower than honeybees (8.7 cM/Mb vs. 37 cM/Mb). Importantly, we find no significant difference in the mutation rates: we estimate for bumblebees a rate of 3.6 × 10−9 per haploid genome per generation (95% confidence intervals 2.38 × 10−9 and 5.37 × 10−9) which is just 5% higher than the estimate that of honeybees. Both genomes have approximately one new mutation per haploid genome per generation. While we find evidence for a direct coupling between recombination and mutation (also seen in honeybees), the effect is so weak as to leave almost no footprint on any between-species differences. The similarity in mutation rates suggests an approximate constancy of the mutation rate in insects.
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影响因子：
3.7
作者：
Amos W
通讯作者：
Amos W
影响因子：
7
作者：
Beye, Martin;Gattermeier, Irene;Page, Robert E., Jr.
通讯作者：
Page, Robert E., Jr.
DOI：
10.1098/rspb.2009.1757
发表时间：
2010-05-07
影响因子：
4.7
作者：
Amos, William
通讯作者：
Amos, William
DOI：
10.1073/pnas.1416622112
发表时间：
2015-02-17
影响因子：
11.1
作者：
Arbeithuber, Barbara;Betancourt, Andrea J.;Tiemann-Boege, Irene
通讯作者：
Tiemann-Boege, Irene
DOI：
10.1073/pnas.1006299107
发表时间：
2010-09-14
影响因子：
11.1
作者：
Cardinal, Sophie;Straka, Jakub;Danforth, Bryan N.
通讯作者：
Danforth, Bryan N.