Nicotinic acid adenine dinucleotide phosphate analogues containing substituted nicotinic acid: effect of modification on Ca(2+) release.
Nicotinic acid adenine dinucleotide phosphate analogues containing substituted nicotinic acid: effect of modification on Ca(2+) release.
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DOI：
10.1021/jm1007209
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发表时间：
2010-11-11
影响因子：
7.3
通讯作者：
Walseth TF
中科院分区：
文献类型：
--
作者：
Jain P;Slama JT;Perez-Haddock LA;Walseth TF
以烟酸腺嘌呤二核苷酸磷酸(NAADP)为原料，利用Aplysia calfornica ADP-核糖环化酶或哺乳动物NAD糖水解酶，在烟酸部分的4位或5位进行取代，合成了烟酸二核苷酸类似物。烟酸的4位取代导致海胆卵匀浆中钙离子释放的激动剂活性丧失，以及[~(32)P]NAADP竞争配体结合分析的活性丧失。相反，几个5-取代的NAADP衍生物表现出很高的结合活性和完全的钙释放激动剂活性。5-叠氮基-NAADP在低浓度下能从海胆卵匀浆中释放钙，并与[~(32)P]NAADP竞争结合，IC50为18 nM，表明该化合物可能是一种潜在的光探针，可用于NAADP受体的特异性标记和鉴定。
Analogs of nicotinic acid adenine dinucleotide phosphate (NAADP) with substitution at either the 4- or the 5-position position of the nicotinic acid moiety have been synthesized from NADP enzymatically using Aplysia californica ADP-ribosyl cyclase or mammalian NAD glycohydrolase. Substitution at the 4-position of the nicotinic acid resulted in the loss of agonist potency for release of Ca2+-ions from sea urchin egg homogenates and in potency for competition ligand binding assays using [32P]NAADP. In contrast, several 5-substituted NAADP derivatives showed high potency for binding and full agonist activity for Ca2+ release. 5-Azido-NAADP was shown to release calcium from sea urchin egg homogenates at low concentration and to compete with [32P]NAADP in a competition ligand binding assay with an IC50 of 18 nM, indicating that this compound might be a potential photoprobe useful for specific labeling and identification of the NAADP receptor.
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影响因子：
9.2
作者：
Bak, J;Billington, RA;Genazzani, AA
通讯作者：
Genazzani, AA
影响因子：
--
作者：
Dowden, James;Berridge, Georgina;Galione, Antony
通讯作者：
Galione, Antony
影响因子：
16.6
作者：
Dowden, J;Moreau, C;Potter, BVL
通讯作者：
Potter, BVL
DOI：
10.1083/jcb.150.3.581
发表时间：
2000-08-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Berg I;Potter BV;Mayr GW;Guse AH
通讯作者：
Guse AH
影响因子：
4.8
作者：
Gasser, Andreas;Bruhn, Soren;Guse, Andreas H.
通讯作者：
Guse, Andreas H.