Development of a high-throughput SARS-CoV-2 antibody testing pathway using dried blood spot specimens.
Development of a high-throughput SARS-CoV-2 antibody testing pathway using dried blood spot specimens.
复制标题
利用干血斑标本建立高通量SARS-CoV-2抗体检测途径。
DOI：
10.1177/0004563220981106
复制
发表时间：
2021-03
影响因子：
2.2
通讯作者：
Jolles S
中科院分区：
文献类型：
--
作者：
Moat SJ;Zelek WM;Carne E;Ponsford MJ;Bramhall K;Jones S;El-Shanawany T;Wise MP;Thomas A;George C;Fegan C;Steven R;Webb R;Weeks I;Morgan BP;Jolles S
严重急性呼吸道综合征冠状病毒2型（SARS-CoV-2）的血清学检测在血清流行病学、恢复期血浆检测、抗体持久性和疫苗研究中发挥作用。目前，SARS-CoV-2血清学是使用静脉穿刺收集的血清/血浆进行的。干血斑（DBS）测试提供了显着的优势，因为它是微创的，避免了静脉穿刺与标本邮寄到实验室。开发了一种利用新生儿筛查实验室基础设施的途径，使用酶联免疫吸附试验检测DBS标本中SARS-CoV-2刺突蛋白受体结合域的IgG抗体。检测了SARS-CoV-2抗体阳性和阴性受试者以及聚合酶链反应阳性受试者的配对血浆和DBS标本。还评价了DBS样本稳定性、血量和穿孔位置的影响。来自抗体阴性（n = 85）和抗体阳性（n = 35）受试者以及聚合酶链反应阳性受试者（n = 11）的DBS标本的平均（SD;范围）光密度（OD）分别为0.14（0.046; 0.03-0.27）、0.98（0.41; 0.31-1.64）和1.12（0.37; 0.49-1.54）。行动值OD >0.28正确分配所有情况。用于比较DBS和血浆测定的加权德明回归得出：y = 0.004041 + 1.005x，r = 0.991，Sy/x 0.171，n = 82。从DBS标本中提取抗体的效率> 99%。DBS样本在环境室温和湿度下可稳定至少28天。在DBS标本中可以可靠地检测到SARS-CoV-2 IgG受体结合域抗体。DBS血清学检测的成本低于需要患者旅行、抽血和医院/诊所资源的护理点或血清/血浆测定; DBS测定的开发对于资源匮乏的环境可能特别重要。
Serological assays for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) have roles in seroepidemiology, convalescent plasma-testing, antibody durability and vaccine studies. Currently, SARS-CoV-2 serology is performed using serum/plasma collected by venepuncture. Dried blood spot (DBS) testing offers significant advantages as it is minimally invasive, avoids venepuncture with specimens being mailed to the laboratory. A pathway utilizing a newborn screening laboratory infrastructure was developed using an enzyme-linked immunosorbent assay to detect IgG antibodies against the receptor-binding domain of the SARS-CoV-2 spike protein in DBS specimens. Paired plasma and DBS specimens from SARS-CoV-2 antibody-positive and -negative subjects and polymerase chain reaction positive subjects were tested. DBS specimen stability, effect of blood volume and punch location were also evaluated. DBS specimens from antibody-negative (n = 85) and -positive (n = 35) subjects and polymerase chain reaction positive subjects (n = 11) had a mean (SD; range) optical density (OD) of 0.14 (0.046; 0.03–0.27), 0.98 (0.41; 0.31–1.64) and 1.12 (0.37; 0.49–1.54), respectively. An action value OD >0.28 correctly assigned all cases. The weighted Deming regression for comparison of the DBS and the plasma assay yielded: y = 0.004041 + 1.005x, r = 0.991, Sy/x 0.171, n = 82. Extraction efficiency of antibodies from DBS specimens was >99%. DBS specimens were stable for at least 28 days at ambient room temperature and humidity. SARS-CoV-2 IgG receptor-binding domain antibodies can be reliably detected in DBS specimens. DBS serological testing offers lower costs than either point of care or serum/plasma assays that require patient travel, phlebotomy and hospital/clinic resources; the development of a DBS assay may be particularly important for resource poor settings.
登录
查看更多内容
影响因子：
4.2
作者：
Mei, JV;Alexander, JR;Hannon, WH
通讯作者：
Hannon, WH
影响因子：
3.7
作者：
Truong Tam Nguyen;Lemee, Veronique;Tuaillon, Edouard
通讯作者：
Tuaillon, Edouard
影响因子：
82.9
作者：
Amanat F;Stadlbauer D;Strohmeier S;Nguyen THO;Chromikova V;McMahon M;Jiang K;Arunkumar GA;Jurczyszak D;Polanco J;Bermudez-Gonzalez M;Kleiner G;Aydillo T;Miorin L;Fierer DS;Lugo LA;Kojic EM;Stoever J;Liu STH;Cunningham-Rundles C;Felgner PL;Moran T;García-Sastre A;Caplivski D;Cheng AC;Kedzierska K;Vapalahti O;Hepojoki JM;Simon V;Krammer F
通讯作者：
Krammer F
影响因子：
--
作者：
Stadlbauer, Daniel;Amanat, Fatima;Krammer, Florian
通讯作者：
Krammer, Florian
影响因子：
9.3
作者：
George, Roanna S.;Moat, Stuart J.
通讯作者：
Moat, Stuart J.