Induction of muscle weakness by local inflammation: an experimental animal model.
Induction of muscle weakness by local inflammation: an experimental animal model.
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DOI：
10.1007/s00011-008-8093-7
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发表时间：
2009-04
影响因子：
6.7
通讯作者：
Quan, N.
中科院分区：
文献类型：
--
作者：
Bicer, S.;Reiser, P. J.;Ching, S.;Quan, N.
本研究的目的是表征骨骼肌对炎症剂酪蛋白诱导的局部炎症的反应。将炎症剂酪蛋白注射到正常成年小鼠的右后肢，并将盐水注射到正常成年小鼠的左后肢，每天一次，连续六天。通过白细胞的免疫组织化学标记监测炎症反应。通过电泳测定肌肉蛋白质水平，并通过等长力测量测定肌肉功能。局部炎症是由酪蛋白引起的，与肌肉中大量中性粒细胞和巨噬细胞的积累有关。这种局部炎症导致比目鱼肌中肌球蛋白重链 (MHC) 亚型表达的变化以及总 MHC 浓度的显着降低。发炎的比目鱼肌的最大抽搐力和强直力显着降低。恢复两周后，比目鱼肌的收缩功能完全恢复，蛋白质浓度恢复，炎症细胞消失。这项研究建立了一个独特而稳健的模型，可以进一步研究局部炎症诱导肌肉蛋白降解、收缩力减少以及随后从这种情况中恢复的机制。
The objective of this study was to characterize the response of skeletal muscle to a localized inflammation induced by the inflammatory agent casein. An inflammatory agent, casein, was injected into the right hindlimb and saline was injected into the left hindlimb of normal adult mice, once daily for six consecutive days. Inflammatory response was monitored by immunohistochemical labeling of leukocytes. Muscle protein levels were determined by electrophoresis and muscle function was determined by isometric force measurements. Local inflammation was induced by casein in association with the accumulation of extensive neutrophils and macrophages in the solues muscle. This local inflammation resulted in a shift in myosin heavy chain (MHC) isoform expression and a significant reduction in total MHC concentration in the soleus. Maximal twitch and tetanic forces were significantly reduced in the inflamed soleus. Contractile function in soleus was fully restored after two weeks of recovery, along with the restoration of protein concentration and the disappearance of inflammatory cells. This study establishes a unique and robust model in which mechanisms of local inflammation induced muscle protein degradation, reduction of contractile force, and subsequent recovery from this condition can be further studied.
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影响因子：
11.2
作者：
Krahn, Martin;Lopez de Munain, Adolfo;Levy, Nicolas
通讯作者：
Levy, Nicolas
DOI：
10.1152/ajpregu.00684.2005
发表时间：
2006-04-01
影响因子：
2.8
作者：
Lang, CH;Krawiec, BJ;Frost, RA
通讯作者：
Frost, RA
影响因子：
4.4
作者：
FERNANDEZSOLA, J;SACANELLA, E;URBANOMARQUEZ, A
通讯作者：
URBANOMARQUEZ, A
DOI：
10.1111/j.1748-1716.1985.tb07776.x
发表时间：
1985-01-01
期刊：
ACTA PHYSIOLOGICA SCANDINAVICA
影响因子：
--
作者：
JENNISCHE, E
通讯作者：
JENNISCHE, E
影响因子：
3.3
作者：
Haddad, F;Zaldivar, F;Adams, GR
通讯作者：
Adams, GR