Glycome analysis of extracellular vesicles derived from human induced pluripotent stem cells using lectin microarray.
Glycome analysis of extracellular vesicles derived from human induced pluripotent stem cells using lectin microarray.
复制标题
DOI：
10.1038/s41598-018-22450-2
复制
发表时间：
2018-03-05
影响因子：
4.6
通讯作者：
Tateno H
中科院分区：
文献类型：
--
作者：
Saito S;Hiemori K;Kiyoi K;Tateno H
葡聚糖是细胞外小泡(EVS)的主要组成成分之一。然而，它们的作用和应用还没有完全被探索。在这里，我们使用高密度凝集素微阵列分析了人诱导多能干细胞(HiPSCs)来源的EV的糖类成分。HiPSC来源的EV与非HIPSC来源的EV的糖链图谱不同。此外，rBC2LCN对HiPSC来源的EV有很强的特异性，但不能与非HiPSCs来源的EV结合。此外，其他HiPSC特异性探针，如抗TRA-1-60、抗SSEA4和抗R-10G，与HiPSC来源的EV显示出特异性的结合，但比rBC2LCN更弱。然后，我们开发了一种使用rBC2LCN和磷脂酰丝氨酸受体TIM4的夹心法来特异性检测HiPSC来源的EV。Tim4-rBC2LCN夹心法可以特异性地检测HiPSC来源的EVS，但不能检测非HiPSC来源的EVS，这表明rBC2LCN也可以用于特异性检测HIPSC来源的EVS。综上所述，我们的发现表明，从HiPSCs衍生的EV保留了HiPSCs特有的糖链签名。EV糖蛋白可能成为鉴定和表征用于再生医学的干细胞的新靶点。
Glycans are one of the major building blocks of extracellular vesicles (EVs). However, their roles and applications have not been completely explored. Here, we analyzed the glycome of EVs derived from human induced pluripotent stem cells (hiPSCs) using high-density lectin microarray. The glycan profiles of hiPSC-derived EVs were different from those of non-hiPSC-derived EVs. Moreover, rBC2LCN that shows specific binding to hiPSCs, showed strong specificity for hiPSC-derived EVs but not non-hiPSCs-derived EVs. Further, other hiPSC-specific probes, such as anti-TRA-1-60, anti-SSEA4, and anti-R-10G, exhibited specific, but weaker binding to hiPSC-derived EVs than rBC2LCN. We then developed a sandwich assay using rBC2LCN and a phosphatidylserine receptor, Tim4, to specifically detect hiPSC-derived EVs. The Tim4–rBC2LCN sandwich assay allowed for specific detection of hiPSC-derived EVs but not non-hiPSC-derived EVs, indicating that rBC2LCN could also be used for the specific detection of hiPSC-derived EVs. Together, our findings demonstrate that the characteristic glycan signature of hiPSCs are retained by EVs derived from them. The EV glycome could be novel targets for the identification and characterization of stem cells for use in regenerative medicine.
登录
查看更多内容
影响因子：
7.3
作者：
Andreu Z;Yáñez-Mó M
通讯作者：
Yáñez-Mó M
影响因子：
4.6
作者：
Tateno H;Onuma Y;Ito Y;Hiemori K;Aiki Y;Shimizu M;Higuchi K;Fukuda M;Warashina M;Honda S;Asashima M;Hirabayashi J
通讯作者：
Hirabayashi J
影响因子：
5.9
作者：
Tateno, Hiroaki;Onuma, Yasuko;Ito, Yuzuru;Minoshima, Fumi;Saito, Sayoko;Shimizu, Madoka;Aiki, Yasuhiko;Asashima, Makoto;Hirabayashi, Jun
通讯作者：
Hirabayashi, Jun
影响因子：
4.6
作者：
Nakai W;Yoshida T;Diez D;Miyatake Y;Nishibu T;Imawaka N;Naruse K;Sadamura Y;Hanayama R
通讯作者：
Hanayama R
影响因子：
5.7
作者：
Sulak, Ondrej;Cioci, Gianluca;Wimmerova, Michaela
通讯作者：
Wimmerova, Michaela