Light microscopic localization of alkaline phosphatase in fetal bovine bone using immunoperoxidase and immunogold-silver staining procedures.
Light microscopic localization of alkaline phosphatase in fetal bovine bone using immunoperoxidase and immunogold-silver staining procedures.
复制标题
使用免疫过氧化物酶和免疫金银染色程序对胎牛骨中的碱性磷酸酶进行光学显微镜定位。
DOI：
10.1177/36.3.3278058
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
H. Anderson
中科院分区：
文献类型：
--
作者：
D. Morris;J. C. Randall;H. Anderson
我们通过使用抗生物素蛋白-生物素-免疫过氧化物酶和免疫金银染色程序将碱性磷酸酶定位于胎牛胫骨的干骺端。使用高碘酸盐赖氨酸-多聚甲醛固定的未脱钙骨的低熔点、石蜡包埋切片进行免疫染色。我们认为，完整胚胎骨与该固定剂的组合以及本研究中使用的免疫组织化学程序可能有助于保留抗原性，从而提高免疫标记的效率。免疫过氧化物酶和免疫金银染色方法产生了类似的碱性磷酸酶定位模式。后者虽然不含二氨基联苯胺等免疫试剂，但必须密切监测，以避免在银增强过程中出现非特异性染色。两种方法都揭示了成骨细胞和骨小梁内衬的类骨区域中酶的浓度。这些结果支持了早期酶细胞化学研究的结果，其中成骨细胞被证明具有显着的碱性磷酸酶活性。
We localized alkaline phosphatase in the metaphyses of fetal bovine tibial bone by use of avidin-biotin-immunoperoxidase and immunogold-silver staining procedures. Low melting-point, paraffin-embedded sections of periodate lysine-paraformaldehyde-fixed undecalcified bone were used for immunostaining. We suggest that the combination of intact embryonic bone with this fixative and the immunohistochemical procedures used in this study may have helped to preserve antigenicity and thus to improve the efficiency of immunolabeling. Similar patterns of alkaline phosphatase localization were produced by the immunoperoxidase and immunogold-silver staining methods. The latter, although free of immunoreagents such as diaminobenzidine, must be monitored closely to avoid nonspecific staining during the silver enhancement procedure. Both methods revealed a concentration of the enzyme in osteoblasts and in areas of osteoid that lined the bone trabeculae. The results support the findings of earlier enzyme cytochemical studies in which osteoblasts were shown to have significant alkaline phosphatase activity.
牛基质囊泡碱性磷酸酶单克隆抗体的开发和交叉反应特性。
影响因子：
4.1
作者：
Oppliger,I;Vaananen,HK;Munoz,PA;Hsu,HH;Morris,DC;Anderson,HC
通讯作者：
Anderson,HC
胎牛骨骺软骨基质囊泡碱性磷酸酶的纯化和部分表征。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hsu,HH;Munoz,PA;Barr,J;Oppliger,I;Morris,DC;Vaananen,HK;Tarkenton,N;Anderson,HC
通讯作者：
Anderson,HC
通过基质囊泡矿化。
DOI：
--
发表时间：
1984
期刊：
Scanning electron microscopy
影响因子：
--
作者：
Anderson,HC
通讯作者：
Anderson,HC