Expression of the Major Vault Protein (MVP) and Cellular Vault Particles in Fish.
Expression of the Major Vault Protein (MVP) and Cellular Vault Particles in Fish.
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DOI：
10.1002/ar.23645
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发表时间：
2017-11
期刊：
影响因子：
--
通讯作者：
Rice CD
中科院分区：
文献类型：
--
作者：
Margiotta AL;Bain LJ;Rice CD
细胞穹窿是普遍存在的13兆Da多亚基核糖核蛋白颗粒，可能在核质运输中发挥作用。70%的穹窿质量由一种100 kDa的蛋白质组成，这是主要的穹窿蛋白质。在人类中，最初被鉴定为转移性肺癌中的肺抗性蛋白的药物抗性相关蛋白最终被证明是先前描述的主要穹窿蛋白。本研究从癍点叉尾鮰中克隆了一个主要穹窿蛋白的部分序列。重组主要穹窿蛋白用于产生识别远亲鱼类物种以及小鼠中全长蛋白的单克隆抗体。主要穹窿蛋白在鱼脾、肝、前肾、肾和鳃中表达，在组织和环境或脉管系统界面处的上皮细胞、巨噬细胞或内皮中一致表达。我们发现，穹窿分布在整个细胞的鱼类淋巴细胞，与核和质膜聚集在一些细胞。将蛋白表达研究扩展到在弗吉尼亚州伊丽莎白河南部分支的Atlantic Wood USA- EPA超级基金站点原位收集的大西洋鳉鱼的肝肿瘤病变。主要穹窿蛋白在这些病变中高度表达，在核膜处具有强烈的染色，类似于已知的人类肝脏肿瘤中MVP表达。此外，主要穹窿蛋白的mRNA表达定量通道鲶鱼卵巢细胞系处理后，不同类别的药物。值得注意的是，mRNA表达是由溴化乙锭诱导的，溴化乙锭损伤DNA。
Cellular vaults are ubiquitous 13 mega Da multi-subunit ribonuceloprotein particles that may have a role in nucleocytoplasmic transport. Seventy percent of the vault’s mass consists of a ≈100 kDa protein, the major vault protein. In humans, a drug resistance –associated protein, originally identified as lung resistance protein in metastatic lung cancer, was ultimately shown to be the previously described major vault protein. In this study, a partial major vault protein sequence was cloned from channel catfish. Recombinant major vault protein was used to generate a monoclonal antibody that recognizes full length protein in distantly related fish species, as well as mice. Major vault protein is expressed in fish spleen, liver, anterior kidney, renal kidney, and gills, with a consistent expression in epithelial cells, macrophages, or endothelium at the interface of the tissue and environment or vasculature. We show that vaults are distributed throughout cells of fish lymphoid cells, with nuclear and plasma membrane aggregations in some cells. Protein expression studies were extended to liver neoplastic lesions in Atlantic killifish collected in situ at the Atlantic Wood USA- EPA superfund site on the southern branch of the Elizabeth River, VA. Major vault protein is highly expressed in these lesions, with intense staining at the nuclear membrane, similar to what is known about MVP expression in human liver neoplasia. Additionally, major vault protein mRNA expression was quantified in channel catfish ovarian cell line following treatment with different classes of pharmacological agents. Notably, mRNA expression is induced by ethidium bromide, which damages DNA.
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DOI：
10.1073/pnas.77.5.2936
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BREITMAN, TR;SELONICK, SE;COLLINS, SJ
通讯作者：
COLLINS, SJ
影响因子：
3.8
作者：
Cheng, SH;Lam, W;Fong, WF
通讯作者：
Fong, WF
影响因子：
6.4
作者：
Ikeda, K;Oka, M;Kohno, S
通讯作者：
Kohno, S
影响因子：
17.1
作者：
Mrazek, Jan;Toso, Daniel;Rome, Leonard H.
通讯作者：
Rome, Leonard H.
DOI：
10.1016/j.cimid.2009.10.002
发表时间：
2010-12
期刊：
Comparative immunology, microbiology and infectious diseases
影响因子：
--
作者：
Marsh MB;Rice CD
通讯作者：
Rice CD