Sibling sRNA RyfA1 Influences Shigella dysenteriae Pathogenesis.
Sibling sRNA RyfA1 Influences Shigella dysenteriae Pathogenesis.
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DOI：
10.3390/genes8020050
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发表时间：
2017-01-26
期刊：
影响因子：
3.5
通讯作者：
Murphy ER
中科院分区：
文献类型：
--
作者：
Fris ME;Broach WH;Klim SE;Coschigano PW;Carroll RK;Caswell CC;Murphy ER
志贺氏菌和其他病原体的小调节 RNA (sRNA) 对于毒力相关基因和过程的调节至关重要。在这里，我们表征了 RyfA1，它是由痢疾杆菌产生的一对 sRNA 的兄弟姐妹之一。与其几乎相同的兄弟分子 RyfA2（预计几乎完全由非致病性物种编码）不同，编码 RyfA1 或 RyfA1 样分子的基因的存在与多种肠道病原体的毒力密切相关。在痢疾杆菌中，RyfA1 的过量产生会对细胞间传播的毒力相关过程以及 ompC 的表达产生负面影响，ompC 是编码对志贺氏菌发病机制重要的主要外膜蛋白的基因。有趣的是，RyfA1 的产生是由第二种 sRNA 控制的，这里称为 RyfB1，这是在痢疾杆菌或任何志贺氏菌属中首次出现一种调节性小 RNA 控制另一种的情况。
Small regulatory RNAs (sRNAs) of Shigella dysenteriae and other pathogens are vital for the regulation of virulence-associated genes and processes. Here, we characterize RyfA1, one member of a sibling pair of sRNAs produced by S. dysenteriae. Unlike its nearly identical sibling molecule, RyfA2, predicted to be encoded almost exclusively by non-pathogenic species, the presence of a gene encoding RyfA1, or a RyfA1-like molecule, is strongly correlated with virulence in a variety of enteropathogens. In S. dysenteriae, the overproduction of RyfA1 negatively impacts the virulence-associated process of cell-to-cell spread as well as the expression of ompC, a gene encoding a major outer membrane protein important for the pathogenesis of Shigella. Interestingly, the production of RyfA1 is controlled by a second sRNA, here termed RyfB1, the first incidence of one regulatory small RNA controlling another in S. dysenteriae or any Shigella species.
影响因子：
14.9
作者：
Yang, Jian;Chen, Lihong;Yu, Jun;Sun, Lilian;Jin, Qi
通讯作者：
Jin, Qi