Combined reflection and transmission microscope for telemedicine applications in field settings.
Combined reflection and transmission microscope for telemedicine applications in field settings.
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DOI：
10.1039/c1lc20169g
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发表时间：
2011-08-21
期刊：
影响因子：
6.1
通讯作者：
Ozcan A
中科院分区：
文献类型：
--
作者：
Biener G;Greenbaum A;Isikman SO;Lee K;Tseng D;Ozcan A
我们展示了一个现场便携式直立和倒置显微镜，可以在反射和透射模式的图像标本。这款紧凑且具有成本效益的双模显微镜仅重约135克（<4.8盎司），利用简单的发光二极管（LED），通过位于物平面上方的分束器立方体照亮感兴趣的样品。然后，该LED照明被两个透镜从待收集的样品部分反射，从而在位于分束器立方体上方的光电传感器阵列上创建样品的反射图像。除此之外，照明光束还部分地透射通过相同的样品，然后将相同物体的无透镜同轴全息图投射到位于分束器立方体下方的第二光电传感器阵列上。通过对所获得的无透镜全息图进行快速数字重建，还创建了同一样品的透射图像（相位和振幅）。我们通过对各种微粒、血涂片以及对应于皮肤组织的组织病理学载玻片进行成像来测试这种现场便携式显微镜的性能。被紧凑，重量轻，成本效益，这种组合的反射和透射显微镜可能是特别有用的远程医疗应用在资源有限的设置。
We demonstrate a field-portable upright and inverted microscope that can image specimens in both reflection and transmission modes. This compact and cost-effective dual-mode microscope weighs only ~135 grams (<4.8 ounces) and utilizes a simple light emitting diode (LED) to illuminate the sample of interest using a beam-splitter cube that is positioned above the object plane. This LED illumination is then partially reflected from the sample to be collected by two lenses, creating a reflection image of the specimen onto an opto-electronic sensor-array that is positioned above the beam-splitter cube. In addition to this, the illumination beam is also partially transmitted through the same specimen, which then casts lensfree in-line holograms of the same objects onto a second opto-electronic sensor-array that is positioned underneath the beam-splitter cube. By rapid digital reconstruction of the acquired lensfree holograms, transmission images (both phase and amplitude) of the same specimen are also created. We tested the performance of this field-portable microscope by imaging various micro-particles, blood smears as well as a histopathology slide corresponding to skin tissue. Being compact, light-weight and cost-effective, this combined reflection and transmission microscope might especially be useful for telemedicine applications in resource limited settings.
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影响因子：
7.4
作者：
Seo, Sungkyu;Isikman, Serhan O.;Sencan, Ikbal;Mudanyali, Onur;Su, Ting-Wei;Bishara, Waheb;Erlinger, Anthony;Ozcan, Aydogan
通讯作者：
Ozcan, Aydogan
影响因子：
6.1
作者：
Bishara W;Sikora U;Mudanyali O;Su TW;Yaglidere O;Luckhart S;Ozcan A
通讯作者：
Ozcan A
影响因子：
6.1
作者：
Balsam, Joshua;Ossandon, Miguel;Rasooly, Avraham
通讯作者：
Rasooly, Avraham
影响因子：
6.1
作者：
Tseng D;Mudanyali O;Oztoprak C;Isikman SO;Sencan I;Yaglidere O;Ozcan A
通讯作者：
Ozcan A
影响因子：
6.1
作者：
Pang, Shuo;Cui, Xiquan;DeModena, John;Wang, Ying Min;Sternberg, Paul;Yang, Changhuei
通讯作者：
Yang, Changhuei