Cell density and airspace patterning in the leaf can be manipulated to increase leaf photosynthetic capacity.
Cell density and airspace patterning in the leaf can be manipulated to increase leaf photosynthetic capacity.
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DOI：
10.1111/tpj.13727
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发表时间：
2017-12
期刊：
影响因子：
--
通讯作者：
Fleming AJ
中科院分区：
文献类型：
--
作者：
Lehmeier C;Pajor R;Lundgren MR;Mathers A;Sloan J;Bauch M;Mitchell A;Bellasio C;Green A;Bouyer D;Schnittger A;Sturrock C;Osborne CP;Rolfe S;Mooney S;Fleming AJ
叶片发育过程中细胞分裂、生长和分离的模式决定了叶片中空气空间的模式和体积。由此产生的细胞物质和空域的平衡预计将显着影响叶片的主要功能，光合作用，但细胞分裂模式影响空域网络和光合作用的方式和程度仍然在很大程度上未被探索。本文通过对叶肉细胞周期相关基因表达的调控，研究了细胞大小与细胞构型、气隙与光合作用的关系。使用microCT成像来量化叶细胞结构和荧光/气体交换分析来测量叶功能，我们表明，拟南芥叶肉细胞密度的增加可用于增加叶光合能力。我们的分析表明，这是通过增加组织密度（减少空气空间的相对体积）和改变叶片内的空气空间分布模式来实现的。我们的研究结果表明，细胞分裂模式影响叶片的光合作用性能，并且有可能通过这种方法来改善光合作用。光合作用的效率受细胞物质（光合作用发生的地方）和空气空间（光合作用所需的二氧化碳通过空气空间传递）的分布影响，但细胞分裂影响这种平衡的方式和程度，因此光合作用在很大程度上仍未被探索。使用转基因的方法，本文表明，这是可能的工程增加光合作用，通过操纵细胞分裂和空域的模式。
The pattern of cell division, growth and separation during leaf development determines the pattern and volume of airspace in a leaf. The resulting balance of cellular material and airspace is expected to significantly influence the primary function of the leaf, photosynthesis, and yet the manner and degree to which cell division patterns affect airspace networks and photosynthesis remains largely unexplored. In this paper we investigate the relationship of cell size and patterning, airspace and photosynthesis by promoting and repressing the expression of cell cycle genes in the leaf mesophyll. Using microCT imaging to quantify leaf cellular architecture and fluorescence/gas exchange analysis to measure leaf function, we show that increased cell density in the mesophyll of Arabidopsis can be used to increase leaf photosynthetic capacity. Our analysis suggests that this occurs both by increasing tissue density (decreasing the relative volume of airspace) and by altering the pattern of airspace distribution within the leaf. Our results indicate that cell division patterns influence the photosynthetic performance of a leaf, and that it is possible to engineer improved photosynthesis via this approach. The efficiency of photosynthesis is influenced by the distribution of cell material (where photosynthesis occurs) and airspace (by which the carbon dioxide for photosynthesis is delivered), yet the manner and degree to which cell division impacts this balance and, thus, photosynthesis remains largely unexplored. Using a transgenic approach, this paper demonstrates that it is possible to engineer increased photosynthesis by manipulating the patterns of cell division and airspace.
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影响因子：
3
作者：
John, Grace P.;Scoffoni, Christine;Sack, Lawren
通讯作者：
Sack, Lawren
DOI：
10.1111/tpj.12342
发表时间：
2013-12
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Dorca-Fornell C;Pajor R;Lehmeier C;Pérez-Bueno M;Bauch M;Sloan J;Osborne C;Rolfe S;Sturrock C;Mooney S;Fleming A
通讯作者：
Fleming A
影响因子：
11.6
作者：
Dewitte, W;Riou-Khamlichi, C;Murray, JAH
通讯作者：
Murray, JAH
DOI：
10.1073/pnas.0603490103
发表时间：
2006-09-26
影响因子：
11.1
作者：
Cloetens, Peter;Mache, Regis;Lerbs-Mache, Silva
通讯作者：
Lerbs-Mache, Silva
影响因子：
4.3
作者：
BROOKS, A;FARQUHAR, GD
通讯作者：
FARQUHAR, GD