Increased leaf mesophyll porosity following transient retinoblastoma-related protein silencing is revealed by microcomputed tomography imaging and leads to a system-level physiological response to the altered cell division pattern.
Increased leaf mesophyll porosity following transient retinoblastoma-related protein silencing is revealed by microcomputed tomography imaging and leads to a system-level physiological response to the altered cell division pattern.
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DOI：
10.1111/tpj.12342
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发表时间：
2013-12
期刊：
影响因子：
--
通讯作者：
Fleming A
中科院分区：
文献类型：
--
作者：
Dorca-Fornell C;Pajor R;Lehmeier C;Pérez-Bueno M;Bauch M;Sloan J;Osborne C;Rolfe S;Sturrock C;Mooney S;Fleming A
植物细胞分裂和生长之间的因果关系是复杂的。虽然细胞周期基因表达的改变经常导致器官生长的改变，但有许多例子表明，尽管组成细胞的大小发生了变化，但对分裂机制的操纵在器官形态水平上导致了有限的结果。一种可能性，这是探索不足，是改变分裂模式所造成的操纵细胞周期基因表达改变器官的生理，这对生长有影响。我们进行了一系列的实验视网膜母细胞瘤相关蛋白（RBR），一个很好的特点调节细胞周期，调查叶生理细胞分裂的改变的结果。我们的方法涉及高分辨率microCT成像和生理分析与瞬时基因诱导系统相结合，为发育生理学的研究提供了一个强大的方法。我们的研究确定了一个新的作用RBR叶肉分化，影响组织孔隙度和叶片内的空气空间的分布。这些数据表明RBR在早期叶发育中的重要性，以及生理适应改变细胞周期基因表达所导致的细胞结构的程度。
The causal relationship between cell division and growth in plants is complex. Although altered expression of cell-cycle genes frequently leads to altered organ growth, there are many examples where manipulation of the division machinery leads to a limited outcome at the level of organ form, despite changes in constituent cell size. One possibility, which has been under-explored, is that altered division patterns resulting from manipulation of cell-cycle gene expression alter the physiology of the organ, and that this has an effect on growth. We performed a series of experiments on retinoblastoma-related protein (RBR), a well characterized regulator of the cell cycle, to investigate the outcome of altered cell division on leaf physiology. Our approach involved combination of high-resolution microCT imaging and physiological analysis with a transient gene induction system, providing a powerful approach for the study of developmental physiology. Our investigation identifies a new role for RBR in mesophyll differentiation that affects tissue porosity and the distribution of air space within the leaf. The data demonstrate the importance of RBR in early leaf development and the extent to which physiology adapts to modified cellular architecture resulting from altered cell-cycle gene expression.
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影响因子：
64.5
作者：
Cruz-Ramírez A;Díaz-Triviño S;Blilou I;Grieneisen VA;Sozzani R;Zamioudis C;Miskolczi P;Nieuwland J;Benjamins R;Dhonukshe P;Caballero-Pérez J;Horvath B;Long Y;Mähönen AP;Zhang H;Xu J;Murray JA;Benfey PN;Bako L;Marée AF;Scheres B
通讯作者：
Scheres B
影响因子：
4.6
作者：
Gutzat, Ruben;Borghi, Lorenzo;Gruissem, Wilhelm
通讯作者：
Gruissem, Wilhelm
影响因子：
11.6
作者：
Ach, RA;Taranto, P;Gruissem, W
通讯作者：
Gruissem, W
影响因子：
7.2
作者：
Horiguchi, Gorou;Fujikura, Ushio;Tsukaya, Hirokazu
通讯作者：
Tsukaya, Hirokazu
影响因子：
11.4
作者：
HEMERLY, A;ENGLER, JD;FERREIRA, P
通讯作者：
FERREIRA, P