Quantitative determination of opioids in whole blood using fully automated dried blood spot desorption coupled to on-line SPE-LC-MS/MS.
Quantitative determination of opioids in whole blood using fully automated dried blood spot desorption coupled to on-line SPE-LC-MS/MS.
复制标题
使用全自动干血点解吸结合在线 SPE-LC-MS/MS 定量测定全血中的阿片类药物。
DOI：
10.1002/dta.1927
复制
发表时间：
2016
影响因子：
2.9
通讯作者：
J. Henion
中科院分区：
文献类型：
--
作者：
R. Verplaetse;J. Henion
阿片类药物是众所周知的，广泛使用的止痛药。已经描述了与血液/血浆相比，阿片样物质在干血斑（DBS）基质中的稳定性增加。DBS技术提供的其他好处包括即时采集、微创微量采样、更经济的运输和方便的储存。目前用于分析微量全血样本中阿片类药物的方法仅限于经典的DBS工作流程，包括DBS卡的繁琐手动打孔，然后进行提取和液相色谱-串联质谱（LC-MS/MS）生物分析。本研究的目的是开发和验证一种全自动在线样品制备程序，用于分析与手指针刺血中阿片类药物检测相关的DBS微量样品。为此，DBS卡的自动流通洗脱之后，在线固相萃取（SPE）和分析LC-MS/MS。选择性，灵敏，准确，可重复的定量在亚治疗，治疗和毒性水平的人血液中的五种代表性阿片类药物。吗啡、可待因、羟考酮、氢可酮的可靠响应范围（R（2）≥0.997）为1 - 500 ng/mL全血;芬太尼为0.1 - 50 ng/mL。日间、日内和基质批间准确度和精密度均小于15%（即使在定量下限（LLOQ）水平下）。该方法成功地用于测量氢可酮及其主要代谢产物去甲氢可酮在人体样本。我们的数据支持DBS采样和自动分析在监测阿片类药物以及其他药物的反兴奋剂和疼痛管理方案中的巨大潜力。
Opioids are well known, widely used painkillers. Increased stability of opioids in the dried blood spot (DBS) matrix compared to blood/plasma has been described. Other benefits provided by DBS techniques include point-of-care collection, less invasive micro sampling, more economical shipment, and convenient storage. Current methodology for analysis of micro whole blood samples for opioids is limited to the classical DBS workflow, including tedious manual punching of the DBS cards followed by extraction and liquid chromatography-tandem mass spectrometry (LC-MS/MS) bioanalysis. The goal of this study was to develop and validate a fully automated on-line sample preparation procedure for the analysis of DBS micro samples relevant to the detection of opioids in finger prick blood. To this end, automated flow-through elution of DBS cards was followed by on-line solid-phase extraction (SPE) and analysis by LC-MS/MS. Selective, sensitive, accurate, and reproducible quantitation of five representative opioids in human blood at sub-therapeutic, therapeutic, and toxic levels was achieved. The range of reliable response (R(2) ≥0.997) was 1 to 500 ng/mL whole blood for morphine, codeine, oxycodone, hydrocodone; and 0.1 to 50 ng/mL for fentanyl. Inter-day, intra-day, and matrix inter-lot accuracy and precision was less than 15% (even at lower limits of quantitation (LLOQ) level). The method was successfully used to measure hydrocodone and its major metabolite norhydrocodone in incurred human samples. Our data support the enormous potential of DBS sampling and automated analysis for monitoring opioids as well as other pharmaceuticals in both anti-doping and pain management regimens.
影响因子：
3.1
作者：
C. Clavijo;J. Thomas;M. Cromie;B. Schniedewind;K. Hoffman;U. Christians;J. Galinkin
通讯作者：
C. Clavijo;J. Thomas;M. Cromie;B. Schniedewind;K. Hoffman;U. Christians;J. Galinkin
影响因子：
--
作者：
Anne Depriest;Brandi L. Puet;A. Holt;A. Roberts;E. Cone
通讯作者：
Anne Depriest;Brandi L. Puet;A. Holt;A. Roberts;E. Cone
影响因子：
4.2
作者：
Griffiths, Rian L.;Dexter, Alex;Cooper, Helen J.
通讯作者：
Cooper, Helen J.