Bone-remodeling transcript levels are independent of perching in end-of-lay white leghorn chickens.
Bone-remodeling transcript levels are independent of perching in end-of-lay white leghorn chickens.
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DOI：
10.3390/ijms16022663
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发表时间：
2015-01-23
影响因子：
5.6
通讯作者：
Rubin DA
中科院分区：
文献类型：
--
作者：
Dale MD;Mortimer EM;Kolli S;Achramowicz E;Borchert G;Juliano SA;Halkyard S;Seitz N;Gatto C;Hester PY;Rubin DA
骨质疏松症是一种骨质疾病，通常导致用于生产供人类食用的鸡蛋的母鸡30%的骨折发生率。骨质疏松症的原因之一是对负重骨骼缺乏机械张力。在传统的笼养母鸡中，没有足够的空间让鸡运动，并对它们的骨骼产生机械张力。一种方法是通过在传统的笼子中增加栖木来增加骨骼的机械应力。本研究主要研究了产蛋末期母鸡（71周龄）与栖木接触后骨重塑的分子机制。我们研究了在发育和重塑过程中积极参与的骨特异性转录本。采用实时定量PCR检测了趾骨、胫骨和股骨中COL2A1（胶原、ⅱ型、α 1）、RANKL（核因子κ b配体受体激活因子）、OPG（骨保护素）、PTHLH （PTH样激素）、PTH1R （PTH/PTHLH 1型受体）、PTH3R （PTH/PTHLH 3型受体）和SOX9 （sly相关高迁移率组）等7种转录本。我们的结果表明，唯一显著的影响是COL2A1（股骨>指骨）骨骼之间的差异。因此，我们得出结论，接触鲈鱼不会改变转录本的表达。此外，由于母鸡已被用作人类骨代谢和骨质疏松症的模型，结果表明，鸡由于机械负荷而引起的骨重塑可能是与哺乳动物模型中涉及的不同途径的产物。
Osteoporosis is a bone disease that commonly results in a 30% incidence of fracture in hens used to produce eggs for human consumption. One of the causes of osteoporosis is the lack of mechanical strain placed on weight-bearing bones. In conventionally-caged hens, there is inadequate space for chickens to exercise and induce mechanical strain on their bones. One approach is to encourage mechanical stress on bones by the addition of perches to conventional cages. Our study focuses on the molecular mechanism of bone remodeling in end-of-lay hens (71 weeks) with access to perches. We examined bone-specific transcripts that are actively involved during development and remodeling. Using real-time quantitative PCR, we examined seven transcripts (COL2A1 (collagen, type II, alpha 1), RANKL (receptor activator of nuclear factor kappa-B ligand), OPG (osteoprotegerin), PTHLH (PTH-like hormone), PTH1R (PTH/PTHLH type-1 receptor), PTH3R (PTH/PTHLH type-3 receptor), and SOX9 (Sry-related high mobility group box)) in phalange, tibia and femur. Our results indicate that the only significant effect was a difference among bones for COL2A1 (femur > phalange). Therefore, we conclude that access to a perch did not alter transcript expression. Furthermore, because hens have been used as a model for human bone metabolism and osteoporosis, the results indicate that bone remodeling due to mechanical loading in chickens may be a product of different pathways than those involved in the mammalian model.
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影响因子：
2.4
作者：
HUGHES, BO;WILSON, S;SMITH, SF
通讯作者：
SMITH, SF
影响因子：
2.4
作者：
McCoy, MA;Reilly, GAC;Kilpatrick, DJ
通讯作者：
Kilpatrick, DJ
DOI：
10.1080/09064709709362394
发表时间：
1997-11-01
影响因子：
0.9
作者：
Abrahamsson, P;Tauson, R
通讯作者：
Tauson, R
影响因子：
4.8
作者：
Rubin, DA;Jüppner, H
通讯作者：
Jüppner, H
影响因子：
4.8
作者：
Saini, Vaibhav;Marengi, Dean A.;Pajevic, Paola Divieti
通讯作者：
Pajevic, Paola Divieti