Interactions between herpes simplex virus DNA-binding proteins.
Interactions between herpes simplex virus DNA-binding proteins.
复制标题
单纯疱疹病毒 DNA 结合蛋白之间的相互作用。
DOI：
--
复制
发表时间：
1984
影响因子：
3.8
通讯作者：
K. Powell
中科院分区：
文献类型：
--
作者：
P. Vaughan;L. Banks;D. Purifoy;K. Powell
针对几种单纯疱疹病毒（HSV）诱导的DNA结合蛋白的单克隆抗体用于研究HSV感染细胞中的蛋白质相互作用。Q1单克隆抗体，这是特异性的HSV-诱导的碱性核酸酶，当在免疫吸附柱中使用时，导致在碱性核酸酶的纯化，其中大量的主要DNA结合蛋白结合。相反，当在亲和层析中使用针对主要DNA结合蛋白的单克隆抗体时，除了主要DNA结合蛋白之外，其他多肽（包括DNA聚合酶和碱性核酸酶）也被洗脱。当使用另一种HSV-2 DNA结合蛋白的单克隆抗体进行实验时，获得了类似的结果。这些结果表明，这些多肽作为HSV DNA复制复合物的一部分相互作用的可能性，并讨论了这一假设。
Monoclonal antibodies directed against several herpes simplex virus (HSV)-induced DNA-binding proteins were used to investigate protein interactions in HSV-infected cells. Q1 monoclonal antibody, which is specific for the HSV-induced alkaline nuclease, when used in an immunoadsorbant column resulted in the purification of the alkaline nuclease, to which large quantities of the major DNA-binding protein were bound. Conversely, when a monoclonal antibody to the major DNA-binding protein was used in affinity chromatography other polypeptides (including the DNA polymerase and alkaline nuclease) were eluted in addition to the major DNA-binding protein. Similar results were obtained when the experiment was performed using a monoclonal antibody to another HSV-2 DNA-binding protein. These results suggest the possibility that these polypeptides interact as part of the HSV DNA replication complex, and this hypothesis is discussed.
DOI：
10.1101/sqb.1983.047.01.077
发表时间：
1982-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
--
作者：
ALBERTS, BM;BARRY, J;KREUZER, KN
通讯作者：
KREUZER, KN