Ultrastructure of the giant cell infiltrate of subcutaneously implanted bone particles in rats and mice.
Ultrastructure of the giant cell infiltrate of subcutaneously implanted bone particles in rats and mice.
复制标题
大鼠和小鼠皮下植入骨颗粒的巨细胞浸润的超微结构。
DOI：
--
复制
发表时间：
1986
影响因子：
--
通讯作者：
Sandy C. Marks
中科院分区：
文献类型：
--
作者：
Steven N. Popoff;Sandy C. Marks
软组织巨细胞和矿化组织巨细胞（破骨细胞）具有明显不同的细胞表面受体和超微结构特征。最近，在皮下环境中去除死骨颗粒被描述为骨吸收的原型，一个主要问题是这些异位骨植入物周围的巨细胞和骨表面破坏过程是否与骨骼中的巨细胞相同。我们比较了年轻正常大鼠和小鼠干骺端骨中巨细胞募集到皮下植入的同基因骨颗粒中的细胞学和超微结构，这些骨颗粒具有破骨细胞的相似特征。植入后2、3和4周骨颗粒表面的巨细胞为多核，具有均匀、非空泡化的细胞质，光镜下骨表面界面不明显。在随机分布的少数细胞中，存在小的胞质空泡，高倍镜下在骨表面附近观察到大的空泡。通过透射电镜观察，在巨细胞的大多数表面上，折叠的膜结构与相邻的细胞形成广泛的交错指状结构是突出的特征。在这些指叉骨表面的情况下，观察到类似于皱褶边界的结构，但当在较低放大倍数或连续切片中检查时，这些区域总是两个不同细胞的一部分。骨颗粒的分解似乎是通过吞噬小块和随后在电子致密细胞质空泡中的细胞内消化。来自这些相同的年轻动物的破骨细胞较小，细胞核较少，细胞质空泡集中在骨表面附近，并且具有特征性的皱褶边界和清晰的区域。这些结果证实了其他人的观点，即天然破骨细胞和死骨颗粒上的多核巨细胞在超微结构和骨表面破坏机制方面明显不同。
The giant cells of soft tissues and those of mineralized tissues (osteoclasts) have distinctly different cell surface receptors and ultrastructural characteristics. Recently, the removal of dead bone particles in a subcutaneous environment has been described as a prototype of bone resorption, and a major issue is whether the giant cells that surround these ectopic bone implants and the processes involved in the disruption of bone surfaces are the same as those in the skeleton. We have compared the cytology and ultrastructure of giant cells recruited to subcutaneously implanted isogeneic bone particles with similar features of osteoclasts in metaphyseal bone of young normal rats and mice. Giant cells on surfaces of bone particles 2, 3, and 4 weeks after implantation were multinucleated, had a homogeneous, nonvacuolated cytoplasm, and had a bone surface interface unremarkable by light microscopy. In a few cells randomly distributed, small cytoplasmic vacuoles were present and large vacuoles were noted next to the bone surface at high magnification. By transmission electron microscopy, folded membrane configurations forming extensive interdigitations with adjacent cells were prominent features on most surfaces of giant cells. In instances where these interdigitations abutted bone surfaces, configuration resembling a ruffled border were noted, but these regions were always part of two different cells when examined at lower magnification or in serial sections. Breakdown of bone particles appeared to be by phagocytosis of small pieces and subsequent intracellular digestion in electron-dense cytoplasmic vacuoles. Osteoclasts from these same young animals were smaller with fewer nuclei, had cytoplasmic vacuoles concentrated next to bone surfaces, and had characteristic ruffled borders and clear zones. These results confirm those of others that native osteoclasts and multinucleated giant cells on dead bone particles are distinctly different with respect to both ultrastructure and mechanism of disruption of bone surfaces.
ia 大鼠新生骨石症治疗的细胞特异性。
DOI：
10.1159/000163172
发表时间：
1983
期刊：
Experimental cell biology
影响因子：
--
作者：
Schneider,GB;Byrnes,JE
通讯作者：
Byrnes,JE
治疗石骨症的细胞特异性：多能造血干细胞的分离和治疗。
影响因子：
4.1
作者：
Schneider,GB
通讯作者：
Schneider,GB
来自未通过骨髓移植治愈的无牙（tl/tl）骨质疏松大鼠的骨颗粒在正常同窝小鼠中被吸收。
DOI：
--
发表时间：
1986
期刊：
Bone and mineral
影响因子：
--
作者：
Anderson,JM;Schmitz,PE;MarksJr,SC
通讯作者：
MarksJr,SC