Unraveling co-translational protein folding: Concepts and methods.
Unraveling co-translational protein folding: Concepts and methods.
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DOI：
10.1016/j.ymeth.2017.11.007
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发表时间：
2018-03-15
期刊：
影响因子：
--
通讯作者：
Komar AA
中科院分区：
文献类型：
--
作者：
Komar AA
核磁共振波谱、冷冻电镜以及单分子和时间分辨荧光方法等技术的进步，正在改变我们在活细胞体内以及重组无细胞翻译系统体外研究共翻译蛋白质折叠的能力。这些方法提供了关于新生多肽链的空间组织和动力学以及共翻译蛋白质折叠动力学的全面信息。这些信息使人们对活细胞中蛋白质折叠过程有了更好的理解，并且应该能够解答该领域中剩余的关键问题，比如在蛋白质合成过程中新生链内何时形成何种结构。最终，使用这些技术的研究将促进蛋白质折叠统一概念的发展，蛋白质折叠这一过程对细胞正常功能和生物体的生存能力至关重要。这篇综述描述了当前分析共翻译蛋白质折叠的方法，重点介绍了一些最近开发的能够实时监测共翻译蛋白质折叠的技术。
Advances in techniques such as nuclear magnetic resonance spectroscopy, cryo-electron microscopy, and single-molecule and time-resolved fluorescent approaches are transforming our ability to study co-translational protein folding both in vivo in living cells and in vitro in reconstituted cell-free translation systems. These approaches provide comprehensive information on the spatial organization and dynamics of nascent polypeptide chains and the kinetics of co-translational protein folding. This information has led to an improved understanding of the process of protein folding in living cells and should allow remaining key questions in the field, such as what structures are formed within nascent chains during protein synthesis and when, to be answered. Ultimately, studies using these techniques will facilitate development of a unified concept of protein folding, a process that is essential for proper cell function and organism viability. This review describes current methods for analysis of co-translational protein folding with an emphasis on some of the recently developed techniques that allow monitoring of co-translational protein folding in real-time.
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影响因子：
16.8
作者：
Cabrita LD;Cassaignau AME;Launay HMM;Waudby CA;Wlodarski T;Camilloni C;Karyadi ME;Robertson AL;Wang X;Wentink AS;Goodsell L;Woolhead CA;Vendruscolo M;Dobson CM;Christodoulou J
通讯作者：
Christodoulou J
DOI：
10.1073/pnas.0903750106
发表时间：
2009-12-29
影响因子：
11.1
作者：
Cabrita, Lisa D.;Hsu, Shang-Te Danny;Christodoulou, John
通讯作者：
Christodoulou, John
影响因子：
5.7
作者：
Chen, Bo;Kaledhonkar, Sandip;Frank, Joachim
通讯作者：
Frank, Joachim
影响因子：
3.4
作者：
Abaskharon, Rachel M.;Gai, Feng
通讯作者：
Gai, Feng
影响因子：
5.6
作者：
Fedorov, AN;Baldwin, TO
通讯作者：
Baldwin, TO