Precision assembly of complex cellular microenvironments using holographic optical tweezers.
Precision assembly of complex cellular microenvironments using holographic optical tweezers.
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DOI：
10.1038/srep08577
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发表时间：
2015-02-26
影响因子：
4.6
通讯作者：
Shakesheff K
中科院分区：
文献类型：
--
作者：
Kirkham GR;Britchford E;Upton T;Ware J;Gibson GM;Devaud Y;Ehrbar M;Padgett M;Allen S;Buttery LD;Shakesheff K
细胞微环境的准确研究受到缺乏可以在足够小的长度尺度上在3D中操纵细胞的技术的限制。构建和操纵多细胞显微结构的能力将有助于更详细地了解发育和干细胞生物学等领域的细胞功能。我们提出了一种基于全息光镊的技术来精确地生成定制的细胞微结构。使用胚胎干细胞，不同几何形状的3D结构被创建并使用水凝胶和细胞-细胞粘附方法稳定。化学微环境的控制是通过从位于这些结构内的聚合物微粒暂时释放特定因子来实现的。还产生了复杂的共培养微环境类似物，以再现成体干细胞龛内发现的结构。基于全息光镊的微操作的应用将使研究人员能够形成具有亚微米精度的细胞，基质和分子的复杂结构，从而对生物微环境产生新的见解。
The accurate study of cellular microenvironments is limited by the lack of technologies that can manipulate cells in 3D at a sufficiently small length scale. The ability to build and manipulate multicellular microscopic structures will facilitate a more detailed understanding of cellular function in fields such as developmental and stem cell biology. We present a holographic optical tweezers based technology to accurately generate bespoke cellular micro-architectures. Using embryonic stem cells, 3D structures of varying geometries were created and stabilized using hydrogels and cell-cell adhesion methods. Control of chemical microenvironments was achieved by the temporal release of specific factors from polymer microparticles positioned within these constructs. Complex co-culture micro-environmental analogues were also generated to reproduce structures found within adult stem cell niches. The application of holographic optical tweezers-based micromanipulation will enable novel insights into biological microenvironments by allowing researchers to form complex architectures with sub-micron precision of cells, matrices and molecules.
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影响因子：
3.8
作者：
De Bank, PA;Kellam, B;Shakesheff, KM
通讯作者：
Shakesheff, KM
影响因子：
14
作者：
Ehrbar, Martin;Rizzi, Simone C.;Lutolf, Matthias P.
通讯作者：
Lutolf, Matthias P.
影响因子：
1.9
作者：
Leach, J;Wulff, K;Padgett, M
通讯作者：
Padgett, M
影响因子：
64.8
作者：
ASHKIN, A;DZIEDZIC, JM;YAMANE, T
通讯作者：
YAMANE, T
影响因子：
3.4
作者：
Guck, J;Ananthakrishnan, R;Käs, J
通讯作者：
Käs, J