Monoamine oxidase A is highly expressed by the human corpus luteum of pregnancy.
Monoamine oxidase A is highly expressed by the human corpus luteum of pregnancy.
复制标题
单胺氧化酶 A 在妊娠期人黄体中高度表达。
DOI：
10.1530/rep-08-0004
复制
发表时间：
2008
期刊：
影响因子：
3.8
通讯作者：
M. Ueda
中科院分区：
文献类型：
--
作者：
Y. Takao;H. Fujiwara;S. Yoshioka;S. Fujii;M. Ueda
为了研究妊娠黄体(CL)的生理特性，我们研制了一种抗人黄体细胞的单抗--人黄体-4。用HCL-4亲和纯化的人妊娠或胎盘CL抗原为61 kDa的蛋白。该抗原蛋白的部分氨基酸序列与人单胺氧化酶A(MAOA，EC1.4.3.4)的氨基酸序列一致。MAOA已被证明能分解儿茶酚胺，据报道，儿茶酚胺可调节CL的黄体功能和各种器官的血管收缩。用HCL-4单抗进行免疫组织化学染色，发现MAOA在妊娠CL的大黄体细胞上呈强阳性表达，在小黄体细胞上呈中等表达。在月经周期的CL中，在大黄体细胞上检测到微弱的MAOA，而在小黄体细胞上检测不到MAOA。Western blotting分析证实MAOA在妊娠CL中高表达。Northern印迹分析也显示MAOA在人CL中有表达，且在妊娠CL中的表达高于月经周期的CL。MAOA在妊娠CL中的表达增加，提示MAOA参与了妊娠CL的功能。
To investigate the physiological characteristics of the corpus luteum (CL) of pregnancy, we raised a mAb, human corpus luteum (HCL)-4, against human luteal cells obtained from CL of pregnancy. The affinity-purified antigen from human CL of pregnancy or placenta using HCL-4 was a 61 kDa protein. The partial amino acid sequence of the antigenic protein was identical to that of human monoamine oxidase A (MAOA, EC1.4.3.4). MAOA has been shown to catabolize catecholamines that were reported to regulate luteal function in CL and vasoconstriction in various organs. Immunohistochemistry using HCL-4 mAb showed that MAOA was intensely expressed on large luteal cells and moderately expressed on small luteal cells in the CL of pregnancy. In the CL of menstrual cycle, MAOA was weakly detected on large luteal cells but not detected at all on small luteal cells. Western blotting analysis confirmed the high expression of MAOA in CL of pregnancy. Northern blot analysis also showed the expression of MAOA mRNA in human CL, and showed that its expression was higher in CL of pregnancy than in CL of menstrual cycle. The increased expression of MAOA in the CL of pregnancy suggests the contribution of MAOA to the function of the CL of pregnancy.
登录
查看更多内容
通过原位杂交研究人类生殖组织中松弛素基因的表达。
DOI：
10.1210/jcem.80.1.7829601
发表时间：
1995
期刊：
The Journal of clinical endocrinology and metabolism.
影响因子：
--
作者：
Bogic,LV;Mandel,M;Bryant-Greenwood,GD
通讯作者：
Bryant-Greenwood,GD
儿茶酚胺诱导的兔黄体腺苷酸环化酶异源脱敏：黄体生成激素反应性的丧失与 G 蛋白功能受损有关。
影响因子：
4.8
作者：
Jena,BP;Abramowitz,J
通讯作者：
Abramowitz,J
DOI：
10.1073/pnas.85.13.4934
发表时间：
1988-07-01
影响因子：
11.1
作者：
BACH, AWJ;LAN, NC;SHIH, JC
通讯作者：
SHIH, JC
儿茶酚胺在调节假孕兔黄体功能中是否发挥生理作用？
影响因子：
3.6
作者：
Gadsby,JE;Keyes,PL;Schwartz,TS;Bill2nd,CH;Lucchesi,B
通讯作者：
Lucchesi,B