The effects of buprenorphine on behaviour in the ACI and BN rat inbred strains
The effects of buprenorphine on behaviour in the ACI and BN rat inbred strains
复制标题
丁丙诺啡对 ACI 和 BN 大鼠近交系行为的影响
DOI：
--
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
H. V. van Lith
中科院分区：
文献类型：
--
作者：
H. Avsaroglu;R. Sommer;L. Hellebrekers;L. van Zutphen;H. V. van Lith
丁丙诺啡是一种局部μ κ激动剂，已被证明能影响动物的自发行为。之前，我们已经证明八月哥本哈根爱尔兰(ACI)/SegHsd和布朗挪威(BN)/RijHsd近交系大鼠对丁丙诺啡的镇痛反应有显著差异。本研究的目的是确定这些菌株对丁丙诺啡的行为反应是否也不同，以便为遗传分析和定位参与这种反应的基因提供额外的参数。本研究选用两株雌雄大鼠（n = 6只/株/性别）。每只大鼠分别在15:00时接受三种治疗方案：(A)无挑战；(B)静脉注射生理盐水；(C)按交叉设计静脉注射丁丙诺啡（0.05 mg/kg）。随后，使用自动登记系统（实验动物行为登记和分析系统™）从15:30至07:00 h测定运动、梳理、饮水和进食行为的相对持续时间（s/h）。ACI大鼠和BN大鼠在无挑战行为上观察到显著的品系差异。ACI大鼠，而不是BN大鼠，对丁丙诺啡治疗有反应，运动水平下降，饮食和饮食行为。同样的处理导致两种菌株的梳理行为增加。在方差分析中，运动和进食的性别差异轻微但显著，但在多重比较中未达到统计学显著水平。本研究的结果强调，在使用行为参数评估丁丙诺啡对大鼠的镇痛作用时，必须考虑到菌株特异性效应的可能性。
Summary Buprenorphine is a partial μ, κ agonist that has been shown to influence spontaneous behaviour in animals. Previously, we have demonstrated significant differences in the analgesic response to buprenorphine between the August Copenhagen Irish (ACI)/SegHsd and the Brown Norway (BN)/RijHsd inbred rat strains. The purpose of this study was to determine whether these strains also differed in their behavioural response to buprenorphine in order to provide an additional parameter for the genetic analysis and localization of genes involved in this response. Male and female rats of both strains were used (n = 6/strain/sex) for this study. Each rat was subjected, respectively, to three treatment regimens at 15:00 h: (A) unchallenged; (B) intravenous saline; (C) intravenous buprenorphine (0.05 mg/kg) according to a crossover design. The relative duration (s/h) of locomotion, grooming, drinking and eating behaviour was subsequently determined from 15:30 to 07:00 h using the automatic registration system, Laboratory Animal Behaviour Registration and Analysis System™. Significant strain differences were observed in unchallenged behaviour between the ACI and the BN rats. ACI rats, but not BN rats, responded to buprenorphine treatment with decreased levels of locomotion, drinking and eating behaviour. The same treatment resulted in an increased grooming behaviour in both strains. Slight but significant sex differences were observed for locomotion and eating in the analysis of variance procedure, but did not reach the level of statistical significance in the multiple comparison procedure. The results of this study emphasize the possibility that strain-specific effects must be taken into account when using behavioural parameters for the assessment of the analgesic effects of buprenorphine in rats.
DOI：
10.1073/pnas.96.14.7744
发表时间：
1999-07-06
影响因子：
11.1
作者：
Mogil, JS
通讯作者：
Mogil, JS