Visualization of mechanical stress-mediated Ca(2+) signaling in the gut using intravital imaging.
Visualization of mechanical stress-mediated Ca(2+) signaling in the gut using intravital imaging.
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DOI：
10.12938/bmfh.2019-054
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Adachi T
中科院分区：
文献类型：
--
作者：
Aihara Y;Fukuda Y;Takizawa A;Osakabe N;Aida T;Tanaka K;Yoshikawa S;Karasuyama H;Adachi T
机械感觉系统与维持肠道内稳态有关，但相关机制的细节很少。最近，我们产生了一个有条件的Ca 2+生物传感器黄色骆驼3.60（YC3.60）表达转基因小鼠模型，并建立了一个五维（5D; x，y，z，时间，和Ca 2+）活体成像系统，用于调查淋巴组织和肠上皮细胞的反应。为了验证这种肠道传感系统，我们可视化了具有特异性YC 3.60表达的Nestin-Cre/YC 3.60flox小鼠中肠神经系统（ENS）细胞的反应。ENS，包括肌间神经丛（奥尔巴赫神经丛）和粘膜下神经丛（迈斯纳神经丛），在该小鼠系中可以可视化而不染色，表明探针产生足够的荧光强度。此外，肌间神经丛表现出Ca 2+信号在没有刺激的痉挛。肠上皮细胞表面的神经末梢也表现出Ca 2+信号，而没有刺激。机械应力诱导短暂的显着的钙离子流在肌间神经丛和肠上皮细胞的Nestin-Cre/YC 3.60和CAG-Cre/YC 3.60线，分别。此外，潜在的TRPM 7抑制剂显示出减弱机械应力介导的Ca 2+信号传导。这些数据表明，本活体成像系统可用于可视化ENS细胞和肠上皮细胞中的机械感觉Ca 2+信号。
Mechanosensory systems have been implicated in the maintenance of gut homeostasis, but details on the related mechanisms are scarce. Recently, we generated a conditional Ca2+ biosensor yellow cameleon 3.60 (YC3.60)-expressing transgenic mouse model and established a five-dimensional (5D; x, y, z, time, and Ca2+) intravital imaging system for investigating lymphoid tissues and enteric epithelial cell responses. To validate this gut-sensing system, we visualized responses of enteric nervous system (ENS) cells in Nestin-Cre/YC3.60flox mice with specific YC3.60 expression. The ENS, including the myenteric (Auerbach’s) and submucous (Meissner’s) plexuses, could be visualized without staining in this mouse line, indicating that the probe produced sufficient fluorescent intensity. Furthermore, the myenteric plexus exhibited Ca2+ signaling during peristalsis without stimulation. Nerve endings on the surface of enteric epithelia also exhibited Ca2+ signaling without stimulation. Mechanical stress induced transient salient Ca2+ flux in the myenteric plexus and in enteric epithelial cells in the Nestin-Cre/YC3.60 and the CAG-Cre/YC3.60 lines, respectively. Furthermore, the potential TRPM7 inhibitors were shown to attenuate mechanical stress-mediated Ca2+ signaling. These data indicate that the present intravital imaging system can be used to visualize mechanosensory Ca2+ signaling in ENS cells and enteric epithelial cells.
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影响因子：
24.5
作者：
Depoortere, Inge
通讯作者：
Depoortere, Inge
影响因子：
4.3
作者：
Jerbi K;Bertrand O
通讯作者：
Bertrand O
影响因子：
3.4
作者：
Kim BJ;Lee GS;Kim HW
通讯作者：
Kim HW
DOI：
10.1073/pnas.96.5.2135
发表时间：
1999-03-02
影响因子：
11.1
作者：
Miyawaki, A;Griesbeck, O;Tsien, RY
通讯作者：
Tsien, RY
影响因子：
7.6
作者：
Kim, J. M.;Choi, S.;Park, K.
通讯作者：
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