A novel ras-related rgp1 gene encoding a GTP-binding protein has reduced expression in 5-azacytidine-induced dwarf rice
A novel ras-related rgp1 gene encoding a GTP-binding protein has reduced expression in 5-azacytidine-induced dwarf rice
复制标题
编码 GTP 结合蛋白的新型 ras 相关 rgp1 基因在 5-氮杂胞苷诱导的矮秆水稻中表达减少
DOI：
10.1007/bf00282470
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
S. Youssefian
中科院分区：
文献类型：
--
作者：
H. Sano;S. Youssefian
5-氮胞苷(5-azacytidine)是一种体内有效的DNA甲基化抑制剂，它能诱导基因组DNA和矮秆植株的去甲基化。通过对cDNA文库的差异筛选，鉴定了受处理影响的基因，随后分离到了ras相关基因rgp1。该基因编码226个氨基酸，相对分子质量为24850，与裂殖酵母中与ras相关的pt3蛋白关系最为密切。在转化的大肠杆菌中表达的rgp1蛋白具有明显的GTP结合活性。在幼苗生长过程中，rgp1在萌发后14d开始表达，在28d~42d达到最大值，此后逐渐下降，直到63d达到与14d苗相同的表达水平。结果表明，在5-氮杂胞苷诱导的矮秆植株及其后代的整个生长过程中，rgp1的表达均显著低于未处理的对照植株。这些结果表明，rgp1的表达可能直接或间接地受到DNA甲基化的影响，rgp1蛋白可能在植物的生长发育中发挥重要作用。
SummaryExposure of normal, tall rice (Oryza sativa) seedlings to 5-azacytidine, a powerful inhibitor of DNA methylation in vivo, induced both demethylation of genomic DNA and dwarf plants. Genes that had been affected by treatment were identified by differential screening of a cDNA library, and a ras-related gene, rgp1, was subsequently isolated. The cDNA of rgp1 was found to encode a deduced protein sequence of 226 amino acids with a relative molecular mass of 24850, which was most closely related to the ras-related ypt3 protein of fission yeast, Shizosaccharomyces pombe. The rgp1 protein, expressed in transformed Escherichia coli, clearly showed GTP-binding activity. During seedling growth, rgp1 expression was first observed 14 days after germination, reaching a maximum level between 28 and 42 days, and gradually decreased thereafter until 63 days when it attained the same level of expression as in 14-day-old seedlings. Expression of rgp1 was found to be markedly reduced throughout the growth period of both 5-azacytidine-induced dwarf plants and their progenies, relative to levels in untreated tall control plants. These results suggest that expression of rgp1 may be influenced, either directly or indirectly, by DNA methylation, and that the rgp1 protein may play an important role in plant growth and development.
登录
查看更多内容
影响因子：
16.6
作者：
M. Barbacid
通讯作者：
M. Barbacid
影响因子：
56.9
作者：
DEVOS, AM;TONG, L;KIM, SH
通讯作者：
KIM, SH
影响因子：
3.5
作者：
COX, GN;FIELDS, C;HIRSH, D
通讯作者：
HIRSH, D
SEC4 的突变分析表明了调节囊泡交通的循环机制。
DOI：
10.1002/j.1460-2075.1989.tb03560.x
发表时间：
1989
期刊：
The EMBO journal
影响因子：
--
作者：
Walworth,NC;Goud,B;Kabcenell,AK;Novick,PJ
通讯作者：
Novick,PJ