Immunologic analysis of mononuclear cells in liver tissues and blood of patients with primary sclerosing cholangitis
Immunologic analysis of mononuclear cells in liver tissues and blood of patients with primary sclerosing cholangitis
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原发性硬化性胆管炎患者肝组织和血液中单个核细胞的免疫学分析
DOI：
10.1002/hep.1840050321
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发表时间：
1985
期刊：
影响因子：
13.5
通讯作者：
D. V. Van Thiel
中科院分区：
文献类型：
--
作者：
T. Whiteside;S. Lasky;L. Si;D. V. Van Thiel
应用抗表面抗原的单克隆抗体，对23例原发性硬化性胆管炎患者肝组织浸润的单个核细胞（MNCs）进行分型和定量。其中12例患者还通过流式细胞术计数了循环T淋巴细胞。血液中T淋巴细胞，尤其是T8+细胞减少。汇管区内的MNCs以T细胞为主（78%）。这些细胞中很少有Tac+或HLA-DR+。总MNC（929 ± 90 vs.对照组106 ± 18）包括11% M1+和8% B1+细胞。汇管区常见T4+和T8+细胞聚集。门静脉T_4/T_8比值为1.27 ± 0.1，与增殖期胆管周围T_4/T_8比值（0.8 ± 0.1）有显著性差异（P < 0.01）。T8+和M5+细胞定位于这些增殖区域并浸润导管上皮。另一方面，T4+细胞聚集在纤维化组织的实质侧，并密集地包围某些小叶内胆管。即使在疾病晚期，每次活检中也可观察到MNCs浸润、导管增生和导管周围纤维化程度的变化。
Using monoclonal antibodies to surface antigens, we typed and quantitated the mononuclear cells (MNCs) infiltrating liver tissues from 23 patients with primary sclerosing cholangitis. The circulating T lymphocytes were also enumerated by flow cytometry in 12 of these patients. In blood, T lymphocytes and especially T8+ cells were decreasd. T cells were the major components of MNCs (78%) in portal tracts. Few of these cells were Tac+ or HLA‐DR+. Total MNCs (929 ± 90 vs. 106 ± 18 in controls) included 11% M1+ and 8% B1+ cells. Aggregates of T4+ and T8+ cells were common in portal areas. The portal T4/T8 ratio of 1.27 ± 0.1 was significantly (p < 0.01) different than that around proliferating bile ductules (0.8 ± 0.1). The T8+ and M5+ cells localized in these areas of proliferation and infiltrated ductal epithelium. On the other hand, T4+ cells accumulated on the parenchymal side of fibrosed tissue and densely surrounded certain intralobular bile ducts. Even late in the disease, variability in the extent of MNCs infiltration, ductal proliferation and periductal fibrosis was seen in each biopsy.
原发性胆汁性肝硬化中 T 细胞亚群的改变。
影响因子：
4.6
作者：
Bhan,AK;Dienstag,JL;Wands,JR;Schlossman,SF;Reinherz,EL
通讯作者：
Reinherz,EL
影响因子：
158.5
作者：
MORECKI, R;GLASER, JH;HORWITZ, MS
通讯作者：
HORWITZ, MS
通过经蝶微腺瘤切除术治疗儿童和青少年库欣病。
DOI：
10.1056/nejm198404053101405
发表时间：
1984
期刊：
The New England journal of medicine
影响因子：
--
作者：
Styne,DM;Grumbach,MM;Kaplan,SL;Wilson,CB;Conte,FA
通讯作者：
Conte,FA