Hepatocarcinogenic potency of mixed and pure enantiomers of trans-7,8-dihydrobenzo[a]pyrene-7,8-diol in trout.
Hepatocarcinogenic potency of mixed and pure enantiomers of trans-7,8-dihydrobenzo[a]pyrene-7,8-diol in trout.
复制标题
鳟鱼中反式 7,8-二氢苯并[a]芘-7,8-二醇的混合和纯对映体的肝癌效力。
DOI：
10.1016/0304-3835(93)90150-8
复制
发表时间：
1993
期刊：
影响因子：
9.7
通讯作者：
Williams,DE
中科院分区：
文献类型：
--
作者：
Kelly,JD;Dutchuk,M;Hendricks,JD;Williams,DE
以虹鳟鱼胚胎和鱼苗为实验材料，研究了反式-7，8-二氢苯并[a]芘-7，8-二醇的致癌活性。用（±）-反式-7，8-二氢苯并[a]芘-7，8-二醇（（±）BP-7，8-DHD）显微注射的胚胎在孵化后9个月发生肝肿瘤。然而，这种暴露方案导致了高死亡率。用0.01 - 1.0 μg（±）BP-7，8-DHD显微注射新孵化的囊鱼苗，产生与剂量有关的肝肿瘤（0 - 13%），与胚胎的结果相似，但没有高死亡率的问题。共注射囊鱼苗与（±）BP-7，8-DHD和β-萘啶酮或四氯化碳显著增强肿瘤反应（约100%）。3倍）。通过将0.5 - 5.0 μg剂量的BP-7，8-DHD的纯（+）和（−）对映体显微注射到囊内，评价了其相对致癌效力。结果表明，（−）对映体的效力是（+）的4 - 18倍。混合癌是观察到的最常见的肝脏肿瘤。这些结果表明，鳟鱼胚胎和囊鱼苗都响应于注射BP-7，8-DHD引发肝癌。与β-萘甲酮或四氯化碳共注射所观察到的显著增强表明，细胞色素P-450依赖性和脂质过氧化依赖性途径都可能参与该化合物的生物活化，推测是通过9，10-位的环氧化。与哺乳动物的情况一样，BP-7，8-DHD的（−）对映体是比（+）对映体更强的致癌物。
The hepatocarcinogenic potency of pure and racemic trans-7,8-dihydrobenzo[a]pyrene-7,8-diol was investigated in embryos and sac-fry rainbow trout. Embryos microinjected with (±)-trans-7,8-dihydrobenzo[a]pyrene-7,8-diol ((±) BP-7,8-DHD) developed liver tumors 9 months after hatching. However, this exposure protocol resulted in high mortalities. Microinjection of newly hatched sac-fry with 0.01 – 1.0 μg of (±) BP-7,8-DHD resulted in a dose-dependent production of liver tumors (0 – 13%) similar to the results with embryos but without the problem of high mortalities. Co-injection of sac-fry with (±) BP-7,8-DHD and either β-naphthoflavone or carbon tetrachloride significantly enhanced the tumor response (approx. 3-fold). The relative carcinogenic potencies of the pure (+) and (−) enantiomers of BP-7,8-DHD were evaluated by microinjection into sac-fry at doses of 0.5 – 5.0 μg. The results demonstrated that the (−) enantiomer was 4 – 18 times more potent than the (+). Mixed carcinomas were the most prevalent liver tumors observed. These results demonstrate that trout embryos and sac-fry are both responsive to hepatocarcinogenesis initiation by injection with BP-7,8-DHD. The marked enhancement seen with coinjection of sac-fry with β-naphthoflavone or carbon tetrachloride suggests that both cytochrome P-450-dependent and lipid peroxidation-dependent pathways could be involved in bioactivation of this compound, presumably through epoxidation at the 9,10-position. As is the case with mammals, the (−) enantiomer of BP-7,8-DHD is a more potent carcinogen than the (+) enantiomer.
登录
查看更多内容
影响因子：
6.8
作者：
HAWKINS, WE;WALKER, WW;LYTLE, JS
通讯作者：
LYTLE, JS
膳食暴露和腹腔注射苯并[a]芘对虹鳟鱼的肝癌致癌性。
DOI：
--
发表时间：
1985
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
Hendricks,JD;Meyers,TR;Shelton,DW;Casteel,JL;Bailey,GS
通讯作者：
Bailey,GS
由来自β-萘黄酮喂养的虹鳟鱼的细胞色素 P-450 的纯化同工酶催化的苯并[a]芘羟化酶。
影响因子：
5.8
作者：
Williams,DE;Buhler,DR
通讯作者：
Buhler,DR
脂质过氧化过程中多环芳烃衍生物代谢为最终致癌物。
DOI：
10.1126/science.6304879
发表时间：
1983
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Dix,TA;Marnett,LJ
通讯作者：
Marnett,LJ
影响因子：
4.7
作者：
ELING, T;CURTIS, J;MARNETT, LJ
通讯作者：
MARNETT, LJ