The differentiation of embryonic stem cells seeded on electrospun nanofibers into neural lineages.
The differentiation of embryonic stem cells seeded on electrospun nanofibers into neural lineages.
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DOI：
10.1016/j.biomaterials.2008.09.046
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发表时间：
2009-01
期刊：
影响因子：
14
通讯作者：
Xia, Younan
中科院分区：
文献类型：
--
作者：
Xie, Jingwei;Willerth, Stephanie M.;Li, Xiaoran;Macewan, Matthew R.;Rader, Allison;Sakiyama-Elbert, Shelly E.;Xia, Younan
关键词：
由于干细胞生物学的进步，胚胎干（ES）细胞在作为胚状体培养时可以被诱导分化成特定的成熟细胞谱系。尽管ES细胞衍生的神经祖细胞的移植已经在小动物模型中被证明对于脊髓损伤修复具有一些成功，但是控制ES细胞分化成复杂的、有活力的、更高级的组织仍然是具有挑战性的。通过向胚状体培养物中加入视黄酸4天，小鼠ES细胞被诱导成为神经祖细胞。在这项研究中，我们研究了使用静电纺丝生物可降解聚合物作为支架，不仅用于增强小鼠ES细胞分化为神经谱系，而且用于促进和引导神经突生长。电纺纤维支架和ES细胞衍生的神经前体细胞的组合可能会导致神经损伤修复的更好策略的发展。
Due to advances in stem cell biology, embryonic stem (ES) cells can be induced to differentiate into a particular mature cell lineage when cultured as embryoid bodies. Although transplantation of ES cells-derived neural progenitor cells has been demonstrated with some success for either spinal cord injury repair in small animal model, control of ES cell differentiation into complex, viable, higher ordered tissues is still challenging. Mouse ES cells have been induced to become neural progenitors by adding retinoic acid to embryoid body cultures for 4 days. In this study, we examine the use of electrospun biodegradable polymers as scaffolds not only for enhancing the differentiation of mouse ES cells into neural lineages but also for promoting and guiding the neurite outgrowth. A combination of electrospun fiber scaffolds and ES cells-derived neural progenitor cells could lead to the development of a better strategy for nerve injury repair.
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影响因子：
14
作者：
Willerth, Stephanie M.;Arendas, Kelly J.;Sakiyama-Elbert, Shelly Elese
通讯作者：
Sakiyama-Elbert, Shelly Elese
DOI：
10.1073/pnas.1735463100
发表时间：
2003-10-28
影响因子：
11.1
作者：
Levenberg, S;Huang, NF;Langer, R
通讯作者：
Langer, R
影响因子：
14
作者：
Johansson, F;Carlberg, P;Kanje, M
通讯作者：
Kanje, M
影响因子：
2.7
作者：
BAIN, G;KITCHENS, D;GOTTLIEB, DI
通讯作者：
GOTTLIEB, DI
影响因子：
1.2
作者：
Willerth, Stephanie M.;Rader, Allison;Sakiyama-Elbert, Shelly E.
通讯作者：
Sakiyama-Elbert, Shelly E.