Peripheral blood microRNAs distinguish active ulcerative colitis and Crohn's disease.
Peripheral blood microRNAs distinguish active ulcerative colitis and Crohn's disease.
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DOI：
10.1002/ibd.21450
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发表时间：
2011-01
影响因子：
4.9
通讯作者：
Kwon, John H.
中科院分区：
文献类型：
--
作者：
Wu, Feng;Guo, Natalie Jia;Tian, Hongying;Marohn, Michael;Gearhart, Susan;Bayless, Theodore M.;Brant, Steven R.;Kwon, John H.
克罗恩病（CD）和溃疡性结肠炎（UC）由病理生理学上不同的失调的免疫应答引起，如通过不同的免疫细胞介质和循环细胞因子表达谱的优势所证明的。microRNAs（miRNAs）是一种小的非编码RNA，作为基因表达的负调控因子，在免疫调节中的作用越来越受到人们的重视。我们假设CD和UC患者中循环免疫细胞的差异通过改变的miRNA表达来反映，并且miRNA表达模式可以将CD和UC与正常健康个体区分开来。外周血从患有活动性CD、非活动性CD、活动性UC、非活动性UC的患者和正常健康成人获得。分离总RNA，使用miRNA微阵列评估miRNA表达，并通过成熟miRNA定量RT-PCR验证。与健康对照相比，在活动性CD患者的血液中，五种miRNA显著增加，两种miRNA（149* 和miRplus-F1065）显著减少。与健康对照相比，在活动性UC患者的血液中，12种miRNA显著增加，并且miRNA-505* 显著降低。与活动性CD患者相比，活动性UC患者血液中10种miRNA显著增加，1种miRNA显著减少。外周血miRNA可用于区分活动性CD和UC与健康对照。这些数据支持CD和UC与不同的循环免疫细胞类型相关的证据，并且外周血miRNA的差异表达可能构成IBD未来诊断测试的基础。
Crohn's disease (CD) and ulcerative colitis (UC) result from pathophysiologically distinct dysregulated immune responses, as evidenced by the preponderance of differing immune cell mediators and circulating cytokine expression profiles. MicroRNAs (miRNAs) are small, noncoding RNAs that act as negative regulators of gene expression and have an increasingly recognized role in immune regulation. We hypothesized that differences in circulating immune cells in CD and UC patients are reflected by altered miRNA expression and that miRNA expression patterns can distinguish CD and UC from normal healthy individuals. Peripheral blood was obtained from patients with active CD, inactive CD, active UC, inactive UC and normal healthy adults. Total RNA was isolated and miRNA expression assessed using miRNA microarray and validated by mature miRNA quantitative RT-PCR. Five miRNAs were significantly increased and two miRNAs (149* and miRplus-F1065) were significantly decreased in the blood of active CD patients as compared to healthy controls. Twelve miRNAs were significantly increased and miRNA-505* was significantly decreased in the blood of active UC patients as compared to healthy controls. Ten miRNAs were significantly increased and one miRNA was significantly decreased in the blood of active UC patients as compared to active CD patients. Peripheral blood miRNAs can be used to distinguish active CD and UC from healthy controls. The data support the evidence that CD and UC are associated with different circulating immune cells types and that the differential expression of peripheral blood miRNAs may form the basis of future diagnostic tests for IBD.
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影响因子：
4.7
作者：
Resnick, Kimberly E.;Alder, Hansjuerg;Cohn, David E.
通讯作者：
Cohn, David E.
影响因子：
--
作者：
Stanczyk, Joanna;Pedrioli, Deena A. Leslie;Kyburz, Diego
通讯作者：
Kyburz, Diego
影响因子：
2.3
作者：
Blumberg, Richard S.
通讯作者：
Blumberg, Richard S.
影响因子：
20.3
作者：
Malumbres, Raquel;Sarosiek, Kristopher A.;Lossos, Izidore S.
通讯作者：
Lossos, Izidore S.
DOI：
10.1111/j.1742-4658.2010.07632.x
发表时间：
2010-05
期刊：
The FEBS journal
影响因子：
--
作者：
Jiang X;Tsitsiou E;Herrick SE;Lindsay MA
通讯作者：
Lindsay MA