E-cigarette aerosol exposure can cause craniofacial defects in Xenopus laevis embryos and mammalian neural crest cells.
E-cigarette aerosol exposure can cause craniofacial defects in Xenopus laevis embryos and mammalian neural crest cells.
复制标题
DOI：
10.1371/journal.pone.0185729
复制
发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Dickinson AJG
中科院分区：
文献类型：
--
作者：
Kennedy AE;Kandalam S;Olivares-Navarrete R;Dickinson AJG
自从2007年电子烟（ECIG）进入美国市场以来，电子烟的受欢迎程度激增。许多人，包括育龄妇女，也认为使用ECIG比传统烟草卷烟更安全，并且在怀孕期间没有危险。然而，很少有研究调查暴露于ECIG对发育中的胚胎的影响，对颅面发育的潜在影响一无所知。因此，我们测试了几种雾化电子烟液体（e-cigAM）在活体颅面模型非洲爪蟾（Xenopus laevis）以及哺乳动物神经嵴细胞系中的影响。结果表明，在胚胎发育期间暴露于电子香烟会诱发多种缺陷，包括两种电子香烟的面部正中裂和面部正中发育不全。详细的面部形态学定量分析表明，尼古丁不是诱发颅面缺损的主要因素，但会加剧其他电子烟液成分的影响。此外，虽然两种不同的电子香烟可能对面部外观产生非常相似的影响，但由于电子香烟成分的差异，可能存在细微的差异。对接触这些特定电子香烟的胚胎的进一步评估显示，颅骨软骨和肌肉缺陷以及面部血液供应减少。最后，在哺乳动物神经嵴细胞系中，血管和软骨分化标志物的表达减少，证实了体内效应。我们的研究首次表明，使用ECIG可能对发育中的胚胎构成潜在危害，并导致颅面出生缺陷。这强调了需要对这种新的流行产品进行更多的测试和监管。
Since electronic cigarette (ECIG) introduction to American markets in 2007, vaping has surged in popularity. Many, including women of reproductive age, also believe that ECIG use is safer than traditional tobacco cigarettes and is not hazardous when pregnant. However, there are few studies investigating the effects of ECIG exposure on the developing embryo and nothing is known about potential effects on craniofacial development. Therefore, we have tested the effects of several aerosolized e-cigarette liquids (e-cigAM) in an in vivo craniofacial model, Xenopus laevis, as well as a mammalian neural crest cell line. Results demonstrate that e-cigAM exposure during embryonic development induces a variety of defects, including median facial clefts and midface hypoplasia in two of e-cigAMs tested e-cigAMs. Detailed quantitative analyses of the facial morphology revealed that nicotine is not the main factor in inducing craniofacial defects, but can exacerbate the effects of the other e-liquid components. Additionally, while two different e-cigAMs can have very similar consequences on facial appearances, there are subtle differences that could be due to the differences in e-cigAM components. Further assessment of embryos exposed to these particular e-cigAMs revealed cranial cartilage and muscle defects and a reduction in the blood supply to the face. Finally, the expression of markers for vascular and cartilage differentiation was reduced in a mammalian neural crest cell line corroborating the in vivo effects. Our work is the first to show that ECIG use could pose a potential hazard to the developing embryo and cause craniofacial birth defects. This emphasizes the need for more testing and regulation of this new popular product.
登录
查看更多内容
影响因子：
--
作者：
Dubey A;Saint-Jeannet JP
通讯作者：
Saint-Jeannet JP
影响因子：
2.5
作者：
Ashford K;Wiggins A;Butler K;Ickes M;Rayens MK;Hahn E
通讯作者：
Hahn E
影响因子：
2.7
作者：
Devotta A;Juraver-Geslin H;Gonzalez JA;Hong CS;Saint-Jeannet JP
通讯作者：
Saint-Jeannet JP
影响因子：
4.6
作者：
Deniz E;Jonas S;Hooper M;N Griffin J;Choma MA;Khokha MK
通讯作者：
Khokha MK
核糖体生物发生因子NOL11是最佳的rDNA转录和颅面发育所必需的。
影响因子：
4.5
作者：
Griffin JN;Sondalle SB;Del Viso F;Baserga SJ;Khokha MK
通讯作者：
Khokha MK