T cell proliferation-augmenting activities of the gene 3 protein derived from a phage library clone with CD80-binding activity.
T cell proliferation-augmenting activities of the gene 3 protein derived from a phage library clone with CD80-binding activity.
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来自具有 CD80 结合活性的噬菌体文库克隆的基因 3 蛋白的 T 细胞增殖增强活性。
DOI：
10.4049/jimmunol.161.12.6622
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发表时间：
1998
影响因子：
4.4
通讯作者：
K. Sugimura
中科院分区：
文献类型：
--
作者：
T. Fukumoto;N. Torigoe;Y. Ito;Y. Kajiwara;K. Sugimura
我们已经分离出一个噬菌体克隆，F2，通过用CTLA 4构象识别单克隆抗体（抗CTLA 4单克隆抗体）淘选噬菌体文库。在F2噬菌体克隆的基因3蛋白中插入了一段独特的15个氨基酸的序列（F2-g3 p）。我们在此表明，1）F2-g3 p被抗CTLA 4 mAb识别，但不被抗CD 28 mAb识别，和2）F2-g3 p与CD 80结合，但不与CD 86结合。表面等离子体共振分析显示F2-g3 p与CD 80强烈结合。F2-g3 p抑制CTLA 4与CD 80的结合，但不抑制CTLA 4与CD 86的结合。相反，F2-g3 p弱抑制CD 28与CD 80的结合。当鸡蛋溶菌酶（HEL）引发的淋巴结细胞在体外F2-g3 p的存在下，用HEL刺激，细胞增殖是高度增强的。在没有抗原刺激的情况下，F2-g3 p不诱导T细胞增殖，表明F2-g3 p的共刺激性质。当F2克隆在加入培养物之前与CD 80-IG预孵育时，F2克隆的T细胞扩增活性被消除，表明CD 80结合参与F2-g3 p介导的免疫增强。因此，F2基序赋予CD 80结合活性和对g3 p的免疫调节功能。
We have isolated a phage clone, F2, by panning a phage library with a CTLA4-conformation recognizing mAb (anti-CTLA4 mAb). The unique sequence of 15 amino acids with an internal disulfide bond was inserted in the gene 3 proteins of F2 phage clone (F2-g3p). We show here that 1) F2-g3p was recognized with anti-CTLA4 mAb but not with anti-CD28 mAb, and 2) F2-g3p bound to CD80 but not to CD86. The surface plasmon resonance analysis showed that F2-g3p strongly bound CD80. F2-g3p inhibited the binding of CTLA4 to CD80 but not to CD86. In contrast, F2-g3p weakly inhibited the binding of CD28 with CD80. When hen egg lysozyme (HEL)-primed lymph node cells were stimulated with HEL in the presence of F2-g3p in vitro, cell proliferation was highly potentiated. In the absence of antigenic stimulation, F2-g3p induced no T cell proliferation, indicating the costimulatory nature of F2-g3p. The T cell-augmenting activity of the F2 clone was eliminated when the F2 clone was preincubated with CD80-Ig before the addition to the cultures, indicating the involvement of CD80-binding in the F2-g3p-mediated immunopotentiation. Thus, the F2 motif conferred CD80-binding activity and an immunoregulatory function to the g3p.
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影响因子：
56.9
作者：
FREEMAN, GJ;GRIBBEN, JG;NADLER, LM
通讯作者：
NADLER, LM
影响因子：
3.5
作者：
PARMLEY, SF;SMITH, GP
通讯作者：
SMITH, GP
影响因子：
4.4
作者：
Krummel, MF;Sullivan, TJ;Allison, JP
通讯作者：
Allison, JP
影响因子：
32.4
作者：
WALUNAS, TL;LENSCHOW, DJ;BLUESTONE, JA
通讯作者：
BLUESTONE, JA