Long Noncoding RNA LINC01133 Promotes the Malignant Behaviors of Renal Cell Carcinoma by Regulating the miR-30b-5p/Rab3D Axis.
Long Noncoding RNA LINC01133 Promotes the Malignant Behaviors of Renal Cell Carcinoma by Regulating the miR-30b-5p/Rab3D Axis.
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DOI：
10.1177/0963689720964413
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发表时间：
2020-01
影响因子：
3.3
通讯作者：
Zhao J
中科院分区：
文献类型：
--
作者：
Zhai X;Wu Y;Wang Z;Zhao D;Li H;Chong T;Zhao J
肾细胞癌（Renal cell carcinoma，RCC）是肾癌中最常见的一种，其发病率呈上升趋势。LINC 01133是一种新的lncRNA，与多种癌症的发生有关。然而，LINC 01133在RCC中的作用尚未报道。因此，在本研究中，我们研究LINC 01133在RCC中的功能。采用实时荧光定量聚合酶链反应（RT-PCR）检测肾癌组织、癌旁组织及肾癌细胞系中LINC 01133的表达水平。结果表明LINC 01133在肾细胞癌组织标本和细胞系中呈高表达。下调LINC 01133可显著抑制肾癌细胞的增殖、迁移和侵袭。进一步的机制研究证明LINC 01133直接与microRNA（miR）-30b-5p相互作用并调节RCC细胞系中miR-30 b-5 p的表达。此外，miR-30 b-5 p在RCC细胞系中表现出肿瘤抑制活性，这是通过靶向Ras相关蛋白Rab-3D（Rab 3D）介导的。体内研究显示LINC 01133敲低抑制裸鼠中的肿瘤生长。综上所述，这些发现表明LINC 01133可能是RCC中通过调节miR-30 b-5 p/Rab 3D轴的癌基因。因此，LINC 01133有可能成为肾细胞癌治疗的潜在靶点。
Renal cell carcinoma (RCC) is the most common type of kidney cancer with rising incidence. Long noncoding RNA (lncRNA) LINC01133 is a novel lncRNA that is involved in the development of several types of cancers. However, the role of LINC01133 in RCC has not been reported. Thus, in this study, we investigated the functions of LINC01133 in RCC. The qualitative real-time polymerase chain reaction analysis was performed to examine the levels of LINC01133 in RCC tissues and adjacent tissues, as well as RCC cell lines. The results showed that LINC01133 was highly expressed in RCC tissue specimens and cell lines. Downregulation of LINC01133 significantly inhibited the proliferation, migration, and invasion of RCC cells. Further mechanistic investigations proved that LINC01133 directly interacted with microRNA (miR)-30b-5p and regulated the miR-30b-5p expression in RCC cell lines. Moreover, miR-30b-5p exhibited tumor-suppressive activity in RCC cell lines, which was mediated by targeting Ras-related protein Rab-3D (Rab3D). In vivo study showed that LINC01133 knockdown suppressed tumor growth in the nude mice. Taken together, these findings indicated that LINC01133 might be an oncogene in RCC through regulation of the miR-30b-5p/Rab3D axis. Thus, LINC01133 might serve as a potential therapeutic target for the treatment of RCC.
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