The L-NAME mouse model of preeclampsia and impact to long-term maternal cardiovascular health.
The L-NAME mouse model of preeclampsia and impact to long-term maternal cardiovascular health.
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DOI：
10.26508/lsa.202201517
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发表时间：
2022-08-05
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
--
作者：
先兆子痫的L-NAME小鼠模型模拟了妊娠期疾病的关键方面，但没有证明先兆子痫后个体心血管疾病的长期风险增加。先兆子痫影响全世界20%-8%的妊娠。它与增加长期母体心血管疾病风险有关。本研究评估了血管收缩剂N（ω）-硝基-L-精氨酸甲酯（L-NAME）在小鼠先兆子痫模型中的作用及其对母体心血管健康的长期影响。在这项研究中，我们发现L-NAME的管理模仿先兆子痫的关键特征，包括血压升高，胎儿和胎盘生长受损，并增加循环内皮素-1（血管收缩剂），可溶性fms样酪氨酸激酶-1（抗血管生成因子）和C反应蛋白（炎症标记物）。分娩后，在怀孕期间接受L-NAME的小鼠恢复，与匹配的对照组相比，在分娩后1、2和4周测得的心血管指数没有明显变化。在10周后交付，从L-NAME小鼠收集的动脉收缩显着比对照组更多的苯肾上腺素。此外，这些小鼠的肾脏Mmp 9：Timp 1和心脏Tnf mRNA表达增加，表明炎症增加。这些研究结果表明，尽管在小鼠中给予L-NAME确实模拟了妊娠期间先兆子痫的关键特征，但它似乎没有模拟先兆子痫后个体中心血管疾病风险的不利增加。
The L-NAME mouse model of preeclampsia mimics key aspects of disease in pregnancy, but does not demonstrate the increased long-term risk of cardiovascular disease seen in individuals following preeclampsia. Preeclampsia affects ∼2–8% of pregnancies worldwide. It is associated with increased long-term maternal cardiovascular disease risk. This study assesses the effect of the vasoconstrictor N(ω)-nitro-L-arginine methyl ester (L-NAME) in modelling preeclampsia in mice, and its long-term effects on maternal cardiovascular health. In this study, we found that L-NAME administration mimicked key characteristics of preeclampsia, including elevated blood pressure, impaired fetal and placental growth, and increased circulating endothelin-1 (vasoconstrictor), soluble fms-like tyrosine kinase-1 (anti-angiogenic factor), and C-reactive protein (inflammatory marker). Post-delivery, mice that received L-NAME in pregnancy recovered, with no discernible changes in measured cardiovascular indices at 1-, 2-, and 4-wk post-delivery, compared with matched controls. At 10-wk post-delivery, arteries collected from the L-NAME mice constricted significantly more to phenylephrine than controls. In addition, these mice had increased kidney Mmp9:Timp1 and heart Tnf mRNA expression, indicating increased inflammation. These findings suggest that though administration of L-NAME in mice certainly models key characteristics of preeclampsia during pregnancy, it does not appear to model the adverse increase in cardiovascular disease risk seen in individuals after preeclampsia.
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DOI：
10.1136/bmj.j3078
发表时间：
2017-07-12
期刊：
BMJ (Clinical research ed.)
影响因子：
--
作者：
Behrens I;Basit S;Melbye M;Lykke JA;Wohlfahrt J;Bundgaard H;Thilaganathan B;Boyd HA
通讯作者：
Boyd HA
影响因子：
12.3
作者：
Amaral, Taline A. S.;Ognibene, Dayane T.;Resende, Angela C.
通讯作者：
Resende, Angela C.
四甲基吡嗪可减轻妊娠大鼠一氧化氮抑制的后果
影响因子：
5.6
作者：
Gu, Shengyi;Shen, Huaxiang;Hua, Xiaolin
通讯作者：
Hua, Xiaolin
影响因子：
2.5
作者：
Bokslag, Anouk;van Weissenbruch, Mirjam;de Groot, Christianne J. M.
通讯作者：
de Groot, Christianne J. M.
影响因子：
4.2
作者：
Helman, Sheridan L.;Wilkins, Sarah J.;Frazer, David M.
通讯作者：
Frazer, David M.