Multilayered microcapsules for the sustained-release of angiogenic proteins from encapsulated cells.
Multilayered microcapsules for the sustained-release of angiogenic proteins from encapsulated cells.
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DOI：
10.1016/j.amjsurg.2010.08.001
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发表时间：
2010-11
影响因子：
3
通讯作者：
Brey, Eric M.
中科院分区：
文献类型：
--
作者：
Khanna, Omaditya;Moya, Monica L.;Greisler, Howard P.;Opara, Emmanuel C.;Brey, Eric M.
具有选择性渗透聚-L-鸟氨酸膜的多层藻酸盐微胶囊可用于将细胞包封在内核中和从外层持续释放血管生成蛋白的双重目的。在这项研究中，我们检查了新型嵌合形式的FGF-1（HB-GAM/FGF-1）从藻酸盐微胶囊外层的包裹和释放。将放射性标记的HB-GAM/FGF-1包封在使用不同藻酸盐条件构建的多层藻酸盐微珠的外层中。定量嵌合体的包封和释放。外层能够包封和释放HB-GAM/FGF-1长达30天。由1%的高甘露糖醛酸含量的海藻酸盐制成的外层提供最快的释放，而1.25%的高古罗糖醛酸含量的海藻酸盐显示最长的释放持续时间。多层海藻酸盐微珠的外层可用于HB-GAM/FGF-1的包封和长期释放。
Multilayered alginate microcapsules with a permselective poly-L-ornithine membrane can be used for the dual purpose of encapsulating cells in the inner core and sustained release of angiogenic proteins from the outer layer. In this study, we examined the encapsulation and release of a novel chimeric form of FGF-1 (HB-GAM/FGF-1) from the outer layer of alginate microcapsules. Radiolabeled HB-GAM/FGF-1 was encapsulated in the outer layer of multilayered alginate microbeads constructed using varying alginate conditions. The encapsulation and release of the chimera was quantified. The outer layer was able to encapsulate and release HB-GAM/FGF-1 for up to thirty days. The outer layer made with 1% alginate of high manuronic acid content provided the fastest release, while 1.25% high guluronic acid content alginate displayed the longest duration of release. The outer layer of multilayered alginate microbeads can be used for the encapsulation and long-term release of HB-GAM/FGF-1.
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影响因子：
4.9
作者：
Khanna, Omaditya;Moya, Monica L.;Opara, Emmanuel C.;Brey, Eric M.
通讯作者：
Brey, Eric M.
影响因子：
15.9
作者：
Ozawa, CR;Banfi, A;Blau, HM
通讯作者：
Blau, HM
影响因子：
7.7
作者：
Davalli, AM;Scaglia, L;Weir, GC
通讯作者：
Weir, GC
影响因子：
7.7
作者：
DIONNE, KE;COLTON, CK;YARMUSH, ML
通讯作者：
YARMUSH, ML
影响因子：
2.2
作者：
Garfinkel, MR;Harland, RC;Opara, EC
通讯作者：
Opara, EC