Differential expression of TRPC channels in the left ventricle of spontaneously hypertensive rats
Differential expression of TRPC channels in the left ventricle of spontaneously hypertensive rats
复制标题
自发性高血压大鼠左心室TRPC通道的差异表达
DOI：
10.1007/s11033-009-9792-z
复制
发表时间：
2010-07
影响因子：
2.8
通讯作者：
Zhang, Yun
中科院分区：
文献类型：
--
作者：
Wang, Rong;Ma, Zhi-yong;Zhang, Kai;Li, Duo-ling;Liu, Fang-fang;Feng, Jin-bo;Li, Li;Zhang, Wei;Zhang, Yun
本研究旨在观察自发性高血压大鼠（SHR）左心室典型瞬时受体电位（TRPC）通道的差异表达。超声心动图研究比较SHR与Wistar-Kyoto大鼠（WKY）的左心室功能，并通过定量实时RT-PCR（qRT-PCR）测定TRPC通道的mRNA水平。进行蛋白质印迹以检查mRNA表达是否与蛋白质表达相对应。与WKY组相比，10周龄SHR组TRPC 4和TRPC 5的mRNA表达显著增加（P= 0.032和P = 0.043），TRPC 4/5蛋白含量也显著增加。SHR室壁中短轴缩短率（mFS）低于WKY（P= 0.016）。此外，TRPC 4/5表达增加与血压升高和mFS降低相关。这些结果表明，TRPC 4和5可能是SHR高血压早期心脏表达的主要亚型。这些通道可能参与了左室收缩功能障碍的发生发展。
This study was designed to identify the differential expression of the canonical transient receptor potential (TRPC) channels in the left ventricle of spontaneously hypertensive rats (SHR). Echocardiography studies were performed to compare the left ventricular function in SHR vs. Wistar-Kyoto rats (WKY), and the mRNA level of the TRPC channels was determined by quantitative real-time RT-PCR (qRT-PCR). Western blots were performed to examine whether the mRNA expression corresponded with the protein expression. Compared with the WKY, the mRNA expression of TRPC4 and TRPC5 was significantly increased in the 10-week-old SHR (P= 0.032 for TRPC4 andP= 0.043 for TRPC5), so did the TRPC4/5 protein content. The midwall fractional shortening (mFS) of SHR was lower than WKY (P= 0.016). Furthermore, increased expression of TRPC4/5 was correlated with both increased blood pressure and decreased mFS. These findings suggest that TRPC4 and 5 seem to be the main subtypes expressed in the heart of the SHR at the beginning period of hypertension. Theses channels may participate in the development of left ventricular systolic dysfunction.
登录
查看更多内容
影响因子：
8.3
作者：
Liu, Daoyan;Yang, Dachun;Tepel, Martin
通讯作者：
Tepel, Martin
DOI：
10.1152/ajplung.00319.2003
发表时间：
2004-04-01
影响因子：
4.9
作者：
Wang, J;Shimoda, LA;Sylvester, JT
通讯作者：
Sylvester, JT
DOI：
10.1016/j.bbamcr.2004.08.015
发表时间：
2004-12-06
影响因子：
5.1
作者：
Vazquez, G;Wedel, BJ;Putney, JW
通讯作者：
Putney, JW
影响因子：
37.8
作者：
SHIMIZU, G;HIROTA, Y;GAASCH, WH
通讯作者：
GAASCH, WH
影响因子：
4.8
作者：
Bush, Erik W.;Hood, David B.;McKinsey, Timothy A.
通讯作者：
McKinsey, Timothy A.