Threshold-seq: a tool for determining the threshold in short RNA-seq datasets.
Threshold-seq: a tool for determining the threshold in short RNA-seq datasets.
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DOI:
10.1093/bioinformatics/btx073
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发表时间:
2017-07-01
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影响因子:
--
通讯作者:
Rigoutsos I
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文献类型:
--
作者:
Magee R;Loher P;Londin E;Rigoutsos I
We present ‘Threshold-seq,’ a new approach for determining thresholds in deep-sequencing datasets of short RNA transcripts. Threshold-seq addresses the critical question of how many reads need to support a short RNA molecule in a given dataset before it can be considered different from ‘background.’ The proposed scheme is easy to implement and incorporate into existing pipelines. Source code of Threshold-seq is freely available as an R package at: http://cm.jefferson.edu/threshold-seq/ Supplementary data are available at Bioinformatics online.
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