Threshold-seq: a tool for determining the threshold in short RNA-seq datasets.

Threshold-seq: a tool for determining the threshold in short RNA-seq datasets.
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DOI:
10.1093/bioinformatics/btx073
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发表时间:
2017-07-01
期刊:
Bioinformatics (Oxford, England)
影响因子:
--
通讯作者:
Rigoutsos I
Rigoutsos I
中科院分区:
其他
文献类型:
--
作者:
Magee R;Loher P;Londin E;Rigoutsos I

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我们提出了一种新的方法,用于确定短RNA转录物深度测序数据集的阈值。Seq解决了一个关键问题,即在一个给定的数据集中,需要多少读段才能支持一个短RNA分子,然后才能被认为与“背景”不同。所提出的方案易于实施并纳入现有管道。Seq的源代码作为R软件包可在http://cm.jefferson.edu/threshold-seq/上免费获得,补充数据可在Bioinformatics在线获得。
We present ‘Threshold-seq,’ a new approach for determining thresholds in deep-sequencing datasets of short RNA transcripts. Threshold-seq addresses the critical question of how many reads need to support a short RNA molecule in a given dataset before it can be considered different from ‘background.’ The proposed scheme is easy to implement and incorporate into existing pipelines. Source code of Threshold-seq is freely available as an R package at: http://cm.jefferson.edu/threshold-seq/ Supplementary data are available at Bioinformatics online.
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