Identification of compounds in red wine that effectively upregulate aquaporin-3 as a potential mechanism of enhancement of skin moisturizing.
Identification of compounds in red wine that effectively upregulate aquaporin-3 as a potential mechanism of enhancement of skin moisturizing.
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DOI：
10.1016/j.bbrep.2020.100864
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发表时间：
2020-12
影响因子：
2.7
通讯作者：
Fukui Y
中科院分区：
文献类型：
--
作者：
Nakamura Y;Tsuchiya T;Hara-Chikuma M;Yasui M;Fukui Y
在之前的一项临床研究中，饮用富含由红酒提取物制成的低聚原花青素（OPCs）饮料的健康日本女性角质层中的水分含量高于对照组。这一发现表明，OPCs可以增加皮肤水分含量。在这项研究中，我们通过测定角化细胞中水通道蛋白-3 （AQP3）的表达水平来阐明红酒葡萄中化合物增加角质层水分含量的机制。通过体外实验，我们证实了红酒培养的正常人表皮角质形成细胞（NHEK）诱导AQP3的表达。此外，补充富含OPC的红酒馏分可以增加AQP3的表达。此外，富含opc的组分上调AQP3的表达发现是一种与没食子酸（GA）结合的黄烷-3-醇，特别是低聚化合物。我们发现ga结合的OPC能够上调AQP3的表达，并且这些化合物在红酒中富集。我们的研究结果提示，红酒提高角质层含水量的机制可能是通过上调表皮角质形成细胞中AQP3的表达。我们之前的临床研究表明，红酒多酚会导致皮肤湿润。我们证明了角质形成细胞中AQP3对皮肤保湿的作用机制。OPCs，尤其是ga结合的OPCs，是上调AQP3的活性化合物。通过AQP3的表达推测OPCs对皮肤的保湿作用机制。
In a previous clinical study, the moisture content in the stratum corneum of healthy Japanese women who consumed a beverage rich in oligomeric proanthocyanidins (OPCs) made from red wine extract was found to be higher than that in the control group. This finding suggested that OPCs can increase skin moisture content. In this study, we determined the expression level of aquaporin-3 (AQP3) in keratinocytes to elucidate the mechanism by which compounds in red wine grape increase moisture content in stratum corneum. Through in vitro studies, we confirmed that normal human epidermal keratinocytes (NHEK) incubated with red wine induced AQP3 expression. Furthermore, the supplementation of red wine fractions enriched in OPC was shown to increase AQP3 expression. Besides, the component of OPC-rich fractions that upregulated AQP3 expression was found to be a gallic acid (GA)-binding flavan-3-ol, particularly oligomeric compounds. We found that GA-binding OPC were able to upregulate AQP3 expression and that these compounds were enriched in red wine. Our findings might suggest that the mechanism of enhancement of moisture content in stratum corneum by red wine might be via the upregulation of AQP3 expression in the epidermal keratinocytes. Our previous clinical study showed red wine polyphenol induced skin moisture. We demonstrated the mechanism of skin moisturization via AQP3 in keratinocytes. OPCs, especially GA-binding, were active compound for upregulation of AQP3. Skin moisturization mechanism by OPCs was suggested via AQP3 expression.
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DOI：
10.2147/hp.s97681
发表时间：
2016
期刊：
Hypoxia (Auckland, N.Z.)
影响因子：
--
作者：
Hoogewijs D;Vogler M;Zwenger E;Krull S;Zieseniss A
通讯作者：
Zieseniss A
影响因子：
--
作者：
Asher GN;Viera AJ;Weaver MA;Dominik R;Caughey M;Hinderliter AL
通讯作者：
Hinderliter AL
影响因子：
4.6
作者：
Wu, Zhouwei;Uchi, Hiroshi;Furue, Masutaka
通讯作者：
Furue, Masutaka
影响因子：
8.8
作者：
Capanoglu, Esra;de Vos, Ric C. H.;Beekwilder, Jules
通讯作者：
Beekwilder, Jules
影响因子：
8.8
作者：
Fujimaki, Takahiro;Mori, Shoko;Fukui, Yuko
通讯作者：
Fukui, Yuko