Circulating miRNA panels for specific and early detection in bladder cancer.
Circulating miRNA panels for specific and early detection in bladder cancer.
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DOI：
10.1111/cas.13856
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发表时间：
2019-01
期刊：
影响因子：
5.7
通讯作者：
Ochiya T
中科院分区：
文献类型：
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作者：
Usuba W;Urabe F;Yamamoto Y;Matsuzaki J;Sasaki H;Ichikawa M;Takizawa S;Aoki Y;Niida S;Kato K;Egawa S;Chikaraishi T;Fujimoto H;Ochiya T
膀胱癌是全球癌症死亡的第九大原因。膀胱癌的主要问题是药物治疗和手术切除后复发率高。尽管传统的筛查方法，如膀胱镜检查、尿细胞学检查和超声检查，已广泛应用于临床，但由于准确性低或侵入性高，这些方法的诊断效果并不令人满意。由于循环微RNA （miRNA）谱最近被报道为液体活检中癌症筛查的一种有吸引力的工具，在这里，我们对392例膀胱癌患者的血清样本进行了全球miRNA谱分析，其中100例非癌症样本和480例其他类型癌症样本作为对照。我们将膀胱癌和对照样本随机分为2个队列，一个训练组（N = 486）和一个验证组（N = 486）。通过比较两组对照，我们在训练组和验证组中确定了特异性miRNA，如miR - 6087，用于诊断膀胱癌。此外，我们发现7种miRNA的组合（7‐miRNA面板：miR‐6087、miR‐6724‐5p、miR‐3960、miR‐1343‐5p、miR‐1185‐1‐3p、miR‐6831‐5p和miR‐4695‐5p）能够以最高的准确度（AUC: 0.97、灵敏度：95%、特异性：87%）区分膀胱癌与非膀胱癌和其他类型的肿瘤。无论膀胱癌的分期和分级，诊断准确率都很高。我们的数据表明，7‐miRNA可能是膀胱癌特异性和早期检测的生物标志物。
Bladder cancer is the 9th leading cause of cancer death worldwide. The major problem in bladder cancer is primarily the high recurrence rate after drug treatment and resection. Although conventional screening methods, such as cystoscopy, urinary cytology and ultrasound sonography, have become widely used in clinical settings, the diagnostic performance of these modalities is unsatisfactory due to low accuracy or high invasiveness. Because circulating micro RNA (miRNA) profiles have recently been reported as an attractive tool for liquid biopsy in cancer screening, here, we performed global miRNA profiling of 392 serum samples of bladder cancer patients with 100 non‐cancer samples and 480 samples of other types of cancer as controls. We randomly classified the bladder cancer and control samples into 2 cohorts, a training set (N = 486) and a validation set (N = 486). By comparing both controls, we identified specific miRNA, such as miR‐6087, for diagnosing bladder cancer in the training and validation sets. Furthermore, we found that a combination of 7 miRNA (7‐miRNA panel: miR‐6087, miR‐6724‐5p, miR‐3960, miR‐1343‐5p, miR‐1185‐1‐3p, miR‐6831‐5p and miR‐4695‐5p) could discriminate bladder cancer from non‐cancer and other types of tumors with the highest accuracy (AUC: .97; sensitivity: 95%; specificity: 87%). The diagnostic accuracy was high, regardless of the stage and grade of bladder cancer. Our data demonstrated that the 7‐miRNA panel could be a biomarker for the specific and early detection of bladder cancer.
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影响因子：
13.6
作者：
Yasui T;Yanagida T;Ito S;Konakade Y;Takeshita D;Naganawa T;Nagashima K;Shimada T;Kaji N;Nakamura Y;Thiodorus IA;He Y;Rahong S;Kanai M;Yukawa H;Ochiya T;Kawai T;Baba Y
通讯作者：
Baba Y
影响因子：
10.6
作者：
Moreau, Michael P.;Bruse, Shannon E.;David-Rus, Richard;Buyske, Steven;Brzustowicz, Linda M.
通讯作者：
Brzustowicz, Linda M.
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影响因子：
--
作者：
Du L;Jiang X;Duan W;Wang R;Wang L;Zheng G;Yan K;Wang L;Li J;Zhang X;Pan H;Yang Y;Wang C
通讯作者：
Wang C
从全基因组 microRNA 分析中鉴定出的血清 microRNA 表达特征可作为膀胱癌诊断和复发的新型非侵入性生物标志物
影响因子：
6.4
作者：
Jiang, Xiumei;Du, Lutao;Wang, Chuanxin
通讯作者：
Wang, Chuanxin
影响因子：
--
作者：
Yokoi A;Yoshioka Y;Hirakawa A;Yamamoto Y;Ishikawa M;Ikeda SI;Kato T;Niimi K;Kajiyama H;Kikkawa F;Ochiya T
通讯作者：
Ochiya T