Unveiling massive numbers of cancer-related urinary-microRNA candidates via nanowires.
Unveiling massive numbers of cancer-related urinary-microRNA candidates via nanowires.
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DOI：
10.1126/sciadv.1701133
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发表时间：
2017-12
期刊：
影响因子：
13.6
通讯作者：
Baba Y
中科院分区：
文献类型：
--
作者：
Yasui T;Yanagida T;Ito S;Konakade Y;Takeshita D;Naganawa T;Nagashima K;Shimada T;Kaji N;Nakamura Y;Thiodorus IA;He Y;Rahong S;Kanai M;Yukawa H;Ochiya T;Kawai T;Baba Y
我们展示了第一个报道的方法，使用纳米线，揭示了大量的癌症相关的尿液microRNA。分析尿细胞外囊泡（EV）中的microRNA（miRNAs）对于实现基于miRNAs的、简单的、非侵入性的早期疾病诊断和及时的医学检查是重要的。然而，从尿液中收集稀释浓度的EV（<0.01体积%）的固有困难阻碍了这些诊断和医学检查的发展。我们提出了一种由锚定到微流体衬底的纳米线组成的器件。该装置能够高效率地收集EV，并原位提取不同序列的各种miRNA（约1000种），这大大超过了通过常规超离心方法提取的物种数量。在缓冲液流动期间锚定到衬底中的纳米线的机械稳定性和EV到纳米线上的静电收集是确保所提出的装置成功的两个关键机制。此外，我们使用我们的方法来识别尿液中的miRNA，这些miRNA不仅可以作为泌尿系统恶性肿瘤（膀胱和前列腺）的癌症生物标志物，还可以作为非泌尿系统恶性肿瘤（肺、胰腺和肝脏）的癌症生物标志物。本装置概念将为实现基于尿液的癌症早期诊断和医学检查的长期目标提供基础。
We demonstrate the first reported methodology using nanowires that unveils massive numbers of cancer-related urinary microRNAs. Analyzing microRNAs (miRNAs) within urine extracellular vesicles (EVs) is important for realizing miRNA-based, simple, and noninvasive early disease diagnoses and timely medical checkups. However, the inherent difficulty in collecting dilute concentrations of EVs (<0.01 volume %) from urine has hindered the development of these diagnoses and medical checkups. We propose a device composed of nanowires anchored into a microfluidic substrate. This device enables EV collections at high efficiency and in situ extractions of various miRNAs of different sequences (around 1000 types) that significantly exceed the number of species being extracted by the conventional ultracentrifugation method. The mechanical stability of nanowires anchored into substrates during buffer flow and the electrostatic collection of EVs onto the nanowires are the two key mechanisms that ensure the success of the proposed device. In addition, we use our methodology to identify urinary miRNAs that could potentially serve as biomarkers for cancer not only for urologic malignancies (bladder and prostate) but also for nonurologic ones (lung, pancreas, and liver). The present device concept will provide a foundation for work toward the long-term goal of urine-based early diagnoses and medical checkups for cancer.
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影响因子：
3.7
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Chiyomaru T;Yamamura S;Zaman MS;Majid S;Deng G;Shahryari V;Saini S;Hirata H;Ueno K;Chang I;Tanaka Y;Tabatabai ZL;Enokida H;Nakagawa M;Dahiya R
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Garnis C
影响因子：
3.7
作者：
Barutta F;Tricarico M;Corbelli A;Annaratone L;Pinach S;Grimaldi S;Bruno G;Cimino D;Taverna D;Deregibus MC;Rastaldi MP;Perin PC;Gruden G
通讯作者：
Gruden G