Bacterial catabolism of membrane phospholipids links marine biogeochemical cycles.
Bacterial catabolism of membrane phospholipids links marine biogeochemical cycles.
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DOI：
10.1126/sciadv.adf5122
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发表时间：
2023-04-28
期刊：
影响因子：
13.6
通讯作者：
Scanlan, David J.
中科院分区：
文献类型：
--
作者：
Westermann, Linda M.;Lidbury, Ian D. E. A.;Li, Chun-Yang;Wang, Ning;Murphy, Andrew R. J.;Ferretjans, Maria del Mar Aguilo;Quareshy, Mussa;Shanmugan, Muralidharan;Torcello-Requena, Alberto;Silvano, Eleonora;Zhang, Yu-Zhong;Blindauer, Claudia A.;Chen, Yin;Scanlan, David J.
在海洋系统中，无机磷酸盐的可用性会限制初级生产，导致细菌和浮游植物利用过多的有机形式。其中包括形成所有细胞的脂质双层以及释放的细胞外囊泡的磷脂。然而，磷脂降解的信息几乎是不存在的，尽管它们的相关性的地球化学循环。在这里，我们确定了完整的分解代谢途径的降解常见的磷脂头基磷酸胆碱（PC）和磷酸乙醇胺（PE）在海洋细菌。使用Phaeopathissp。MED 193作为一个模型，我们提供的遗传和生化证据表明，磷脂的胞外水解释放含氮底物乙醇胺和胆碱。乙醇胺（EtoX）和胆碱（BetT）的转运蛋白在全球海洋的整个水柱中普遍存在并高度表达，这突出了磷脂，特别是PE原位催化剂的重要性。因此，在磷脂代谢之后，乙醇胺和胆碱降解途径的分解代谢活化特异性地连接并因此联合磷、氮和碳循环。P-依赖性磷脂催化剂提供了N、C和能量的宝贵来源，从而连接主要的生物地球化学循环。
In marine systems, the availability of inorganic phosphate can limit primary production leading to bacterial and phytoplankton utilization of the plethora of organic forms available. Among these are phospholipids that form the lipid bilayer of all cells as well as released extracellular vesicles. However, information on phospholipid degradation is almost nonexistent despite their relevance for biogeochemical cycling. Here, we identify complete catabolic pathways for the degradation of the common phospholipid headgroups phosphocholine (PC) and phosphorylethanolamine (PE) in marine bacteria. Using Phaeobacter sp. MED193 as a model, we provide genetic and biochemical evidence that extracellular hydrolysis of phospholipids liberates the nitrogen-containing substrates ethanolamine and choline. Transporters for ethanolamine (EtoX) and choline (BetT) are ubiquitous and highly expressed in the global ocean throughout the water column, highlighting the importance of phospholipid and especially PE catabolism in situ. Thus, catabolic activation of the ethanolamine and choline degradation pathways, subsequent to phospholipid metabolism, specifically links, and hence unites, the phosphorus, nitrogen, and carbon cycles. P-dependent phospholipid catabolism provides a valuable source of N, C and energy thereby connecting major biogeochemical cycles.
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DOI：
10.3390/life11020152
发表时间：
2021-02-17
期刊：
Life (Basel, Switzerland)
影响因子：
--
作者：
Biddanda B;Dila D;Weinke A;Mancuso J;Villar-Argaiz M;Medina-Sánchez JM;González-Olalla JM;Carrillo P
通讯作者：
Carrillo P
影响因子：
28.3
作者：
Christie-Oleza JA;Sousoni D;Lloyd M;Armengaud J;Scanlan DJ
通讯作者：
Scanlan DJ
影响因子：
16.6
作者：
Bisson C;Adams NBP;Stevenson B;Brindley AA;Polyviou D;Bibby TS;Baker PJ;Hunter CN;Hitchcock A
通讯作者：
Hitchcock A
影响因子：
64.8
作者：
Amin, S. A.;Hmelo, L. R.;Armbrust, E. V.
通讯作者：
Armbrust, E. V.
影响因子：
2.8
作者：
Frka, Sanja;Gasparovic, Blazenka;Kozarac, Zlatica
通讯作者：
Kozarac, Zlatica