The molecular basis of phosphite and hypophosphite recognition by ABC-transporters.
The molecular basis of phosphite and hypophosphite recognition by ABC-transporters.
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DOI：
10.1038/s41467-017-01226-8
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发表时间：
2017-11-23
影响因子：
16.6
通讯作者：
Hitchcock A
中科院分区：
文献类型：
--
作者：
Bisson C;Adams NBP;Stevenson B;Brindley AA;Polyviou D;Bibby TS;Baker PJ;Hunter CN;Hitchcock A
无机磷是人体必需营养磷的主要生物可利用形式。然而，大多数自然栖息地的磷酸盐浓度低到足以限制微生物的生长。在磷酸盐耗尽的条件下，一些细菌利用亚磷酸盐和次亚磷酸盐作为磷的替代来源，但减少从环境中获取磷的分子基础还不完全清楚。在这里，我们介绍了细菌亚磷酸盐和次磷酸盐ATP结合盒转运体的周质结合蛋白的晶体结构和配体结合亲和力。我们揭示了亚磷酸盐和次亚磷酸盐的专一性是空间选择和P-H…存在的组合的结果配体与配体结合口袋中保守的芳香族残基之间的π相互作用。高亲和力和特定转运体的特征对海洋磷氧化还原循环具有影响，并可能有助于亚磷酸盐在生物技术、工业和农业应用中作为替代磷源的使用。一些细菌可以利用无机亚磷酸盐和次亚磷酸盐作为无机磷的来源。在这里，作者报告了与这些还原的磷物种结合的周质蛋白的晶体结构，并表明P-H…配体和结合部位之间的π相互作用决定了它们的特异性。
Inorganic phosphate is the major bioavailable form of the essential nutrient phosphorus. However, the concentration of phosphate in most natural habitats is low enough to limit microbial growth. Under phosphate-depleted conditions some bacteria utilise phosphite and hypophosphite as alternative sources of phosphorus, but the molecular basis of reduced phosphorus acquisition from the environment is not fully understood. Here, we present crystal structures and ligand binding affinities of periplasmic binding proteins from bacterial phosphite and hypophosphite ATP-binding cassette transporters. We reveal that phosphite and hypophosphite specificity results from a combination of steric selection and the presence of a P-H…π interaction between the ligand and a conserved aromatic residue in the ligand-binding pocket. The characterisation of high affinity and specific transporters has implications for the marine phosphorus redox cycle, and might aid the use of phosphite as an alternative phosphorus source in biotechnological, industrial and agricultural applications. Some bacteria can use inorganic phosphite and hypophosphite as sources of inorganic phosphorus. Here, the authors report crystal structures of the periplasmic proteins that bind these reduced phosphorus species and show that a P-H…π interaction between the ligand and binding site determines their specificity.
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影响因子：
5.6
作者：
Alicea I;Marvin JS;Miklos AE;Ellington AD;Looger LL;Schreiter ER
通讯作者：
Schreiter ER
影响因子：
13.8
作者：
Loera-Quezada MM;Leyva-González MA;Velázquez-Juárez G;Sanchez-Calderón L;Do Nascimento M;López-Arredondo D;Herrera-Estrella L
通讯作者：
Herrera-Estrella L
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
DOI：
10.1093/bioinformatics/bts199
发表时间：
2012-06-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Kearse M;Moir R;Wilson A;Stones-Havas S;Cheung M;Sturrock S;Buxton S;Cooper A;Markowitz S;Duran C;Thierer T;Ashton B;Meintjes P;Drummond A
通讯作者：
Drummond A