Role of capsaicin-sensitive sensory nerves in acid-induced bicarbonate secretion in rat stomach
Role of capsaicin-sensitive sensory nerves in acid-induced bicarbonate secretion in rat stomach
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辣椒素敏感感觉神经在酸诱导大鼠胃碳酸氢盐分泌中的作用
DOI：
10.1007/bf01296432
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发表时间：
1992
影响因子：
3.1
通讯作者：
S. Okabe
中科院分区：
文献类型：
--
作者：
K. Takeuchi;K. Ueshima;J. Matsumoto;S. Okabe
在麻醉大鼠离体胃中，观察辣椒素敏感传入神经对粘膜酸化引起的碱性分泌反应的影响。将胃固定在Lucite小室上，在无酸分泌的情况下(奥美拉唑60 mg/kg，腹腔注射)灌流生理盐水(pH 4.5)，同时监测胃腔pH和跨粘膜电位差(Pd)。在此条件下，胃黏膜对前列腺素E_2(PGE_2：300μg/kg)静脉感染和粘膜酸化(0.2N HCl处理10min)的反应是pH显著升高，PD略有下降，HCO_3−产量分别为9.2±0.7μ和8.4±0.8μ。预先皮下注射消炎痛(5 mg/kg，皮下)或皮下注射辣椒素(总剂量：100 mg/kg)后化学去传入均可显著抑制pH升高和HCO3−反应，而PGE_2引起的上述反应在上述两种处理后均无明显变化。另一方面，粘膜应用辣椒素(0.3-6 mg/ml)以浓度相关的方式增加管腔pH和HCO3−输出，这种作用也被吲哚美辛或辣椒素敏感感觉神经元的化学去传入显著减弱。这些结果表明，辣椒素敏感的感觉神经除了内源性PGs外，可能还参与了酸诱导胃内HCO3−分泌的机制，这两条通路可能在刺激过程中的某个地方相互作用。
The effect of capsaicin-sensitive afferent nerves on the alkaline secretory response induced by mucosal acidification was investigated in theex vivo stomachs of anesthetized rats. The stomach was mounted on a Lucite chamber and perfused with saline (pH 4.5) in the absence of acid secretion (omeprazole pretreatment: 60 mg/kg, intraperitoneal), and luminal pH and transmucosal potential difference (PD) were monitored simultaneously. Under these conditions the gastric mucosa responded to intravenous infection of prostaglandin E2 (PGE2: 300 μg/kg) and mucosal acidification (0.2 N HCl for 10 min) by a significant increase of pH with a slight decrease of PD; the HCO3− output was 9.2±0.7 μmol and 8.4±0.8 μmol, respectively. The increased pH and HCO3− responses were significantly inhibited by prior administration of indomethacin (5 mg/kg, subcutaneously) or chemical deafferentation following capsaicin injections (total dose: 100 mg/kg, subcutaneously), whereas those induced by PGE2 remained unchanged after either treatment. On the other hand, the mucosal application of capsaicin (0.3–6 mg/ml) increased the luminal pH and HCO3− output in a concentration-related manner, and this action was also significantly attenuated by either indomethacin or chemical deafferentation of capsaicin-sensitive sensory neurons. These results suggest that capsaicin-sensitive sensory nerves may be involved in the mechanism of acid-induced HCO3− secretion in the stomach, in addition to endogenous PGs, and these two pathways may interact somewhere in the stimulatory process.
影响因子：
21.1
作者：
S. H. Buck;T. Burks
通讯作者：
S. H. Buck;T. Burks
剖腹手术诱导的胃对乙醇损伤的保护是由辣椒素敏感的感觉神经元介导的。
影响因子：
29.4
作者：
Yonei,Y;Holzer,P;Guth,PH
通讯作者：
Guth,PH