Involvement of a velvet protein FgVeA in the regulation of asexual development, lipid and secondary metabolisms and virulence in Fusarium graminearum.
Involvement of a velvet protein FgVeA in the regulation of asexual development, lipid and secondary metabolisms and virulence in Fusarium graminearum.
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DOI：
10.1371/journal.pone.0028291
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Ma Z
中科院分区：
文献类型：
--
作者：
Jiang J;Liu X;Yin Y;Ma Z
天鹅绒蛋白，VeA，参与多种细胞过程的调节。在这项研究中，我们探讨了FgVeA在小麦赤霉病病原菌，禾谷镰刀菌，使用基因置换策略的功能。FgVEA缺失突变体表现出减少气生菌丝的形成，疏水性，和脱氧雪腐镰刀菌烯醇（DON）的生物合成。FgVEA基因的缺失导致分生孢子产量增加，但分生孢子萌发延迟。致病性测定表明，该突变体对开花小麦的致病力降低。对各种胁迫的敏感性试验表明，FgVEA缺失突变体对渗透胁迫和细胞壁破坏剂的抗性增加，但对异菌脲和咯菌腈杀菌剂的敏感性增加。超微结构和组织化学分析表明，分生孢子的FgVeA缺失突变体含有异常高数量的大脂滴，这是在协议的观察，突变体积累了较高的基础水平的甘油比野生型祖。在FgVEA突变体中的基因表达的系列分析（SAGE）证实FgVeA参与各种细胞过程。此外，本研究还通过酵母双杂交技术鉴定了6个与FgVeA相互作用的蛋白。这些结果表明FgVeA在F.禾谷早熟禾
The velvet protein, VeA, is involved in the regulation of diverse cellular processes. In this study, we explored functions of FgVeA in the wheat head blight pathogen, Fusarium graminearum,using a gene replacement strategy. The FgVEA deletion mutant exhibited a reduction in aerial hyphae formation, hydrophobicity, and deoxynivalenol (DON) biosynthesis. Deletion of FgVEA gene led to an increase in conidial production, but a delay in conidial germination. Pathogencity assays showed that the mutant was impaired in virulence on flowering wheat head. Sensitivity tests to various stresses exhibited that the FgVEA deletion mutant showed increased resistance to osmotic stress and cell wall-damaging agents, but increased sensitivity to iprodione and fludioxonil fungicides. Ultrastructural and histochemical analyses revealed that conidia of FgVeA deletion mutant contained an unusually high number of large lipid droplets, which is in agreement with the observation that the mutant accumulated a higher basal level of glycerol than the wild-type progenitor. Serial analysis of gene expression (SAGE) in the FgVEA mutant confirmed that FgVeA was involved in various cellular processes. Additionally, six proteins interacting with FgVeA were identified by yeast two hybrid assays in current study. These results indicate that FgVeA plays a critical role in a variety of cellular processes in F. graminearum.
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