Complex morphology and functional dynamics of vital murine intestinal mucosa revealed by autofluorescence 2-photon microscopy.
Complex morphology and functional dynamics of vital murine intestinal mucosa revealed by autofluorescence 2-photon microscopy.
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DOI：
10.1007/s00418-011-0905-0
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发表时间：
2012-03
影响因子：
2.3
通讯作者：
Gebert, Andreas
中科院分区：
文献类型：
--
作者：
Klinger, Antje;Orzekowsky-Schroeder, Regina;von Smolinski, Dorthe;Blessenohl, Maike;Schueth, Anna;Koop, Norbert;Huettmann, Gereon;Gebert, Andreas
胃肠道粘膜是由具有复杂细胞功能的多种细胞类型组成的动态组织。由于缺乏合适的模型系统，对活肠粘膜的研究受到阻碍。在这里，我们提出了一种新的动物模型，使高分辨率的三维成像的重要小鼠肠道麻醉小鼠。使用活体自体荧光双光子（A2 P）显微镜，我们研究了编排的相互作用的肠上皮细胞，杯状细胞，肠内分泌细胞和刷细胞与其他细胞成分的小肠粘膜在几个小时内在亚细胞分辨率和三维。剧烈运动的淋巴细胞及其与固有层组成部分的相互作用进行了检查和定量分析。核和凝集素染色允许同时表征自发荧光和承认的染料，并产生额外的光谱信息，这是至关重要的复杂的肠粘膜的解释。这种新的活体方法提供了对小肠生理学的详细了解，特别是为研究接近生理条件下的细胞动力学打开了一个新的窗口。本文的在线版本（doi：10.1007/s 00418 -011-0905-0）包含补充材料，可供授权用户使用。
The mucosa of the gastrointestinal tract is a dynamic tissue composed of numerous cell types with complex cellular functions. Study of the vital intestinal mucosa has been hampered by lack of suitable model systems. We here present a novel animal model that enables highly resolved three-dimensional imaging of the vital murine intestine in anaesthetized mice. Using intravital autofluorescence 2-photon (A2P) microscopy we studied the choreographed interactions of enterocytes, goblet cells, enteroendocrine cells and brush cells with other cellular constituents of the small intestinal mucosa over several hours at a subcellular resolution and in three dimensions. Vigorously moving lymphoid cells and their interaction with constituent parts of the lamina propria were examined and quantitatively analyzed. Nuclear and lectin staining permitted simultaneous characterization of autofluorescence and admitted dyes and yielded additional spectral information that is crucial to the interpretation of the complex intestinal mucosa. This novel intravital approach provides detailed insights into the physiology of the small intestine and especially opens a new window for investigating cellular dynamics under nearly physiological conditions. The online version of this article (doi:10.1007/s00418-011-0905-0) contains supplementary material, which is available to authorized users.
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影响因子：
6.1
作者：
Reséndiz-Albor, AA;Esquivel, R;Moreno-Fierros, L
通讯作者：
Moreno-Fierros, L
DOI：
10.1084/jem.182.2.325
发表时间：
1995-08-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Peters PJ;Raposo G;Neefjes JJ;Oorschot V;Leijendekker RL;Geuze HJ;Ploegh HL
通讯作者：
Ploegh HL
DOI：
10.1073/pnas.0708425104
发表时间：
2007-12-04
影响因子：
11.1
作者：
Skala, Melissa C.;Riching, Kristin M.;Ramanujam, Nirmala
通讯作者：
Ramanujam, Nirmala
影响因子：
2.4
作者：
MARCO, AJ;DOMINGO, M;DOMINGUEZ, L
通讯作者：
DOMINGUEZ, L
影响因子：
15.9
作者：
Smythies, LE;Sellers, M;Smith, PD
通讯作者：
Smith, PD