The Impact of Enzyme Orientation and Electrode Topology on the Catalytic Activity of Adsorbed Redox Enzymes.
The Impact of Enzyme Orientation and Electrode Topology on the Catalytic Activity of Adsorbed Redox Enzymes.
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DOI：
10.1016/j.electacta.2013.01.153
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发表时间：
2013-11-01
影响因子：
6.6
通讯作者：
Jeuken, Lars J. C.
中科院分区：
文献类型：
--
作者：
McMillan, Duncan G. G.;Marritt, Sophie J.;Kemp, Gemma L.;Gordon-Brown, Piers;Butt, Julea N.;Jeuken, Lars J. C.
关键词：
它是公认的，电极的结构细节和它们与吸附的酶的相互作用的影响的界面电子转移速率。然而，对于纳米结构的电极，其结构也可能影响吸附的酶附近的底物通量，从而影响催化活性。此外，对于转化大分子底物的酶，酶的取向可能决定了吸附的酶和底物之间相互作用的性质，从而决定了催化速率。实质上，电极可以阻碍底物接近酶的活性位点。我们已经测试了这些可能性，通过研究吸附在拓扑结构不同的电极材料上的两种酶的催化性能。大肠杆菌NrfA，亚硝酸盐还原酶，吸附在介孔，纳米晶SnO 2电极。来自希瓦氏菌（Shewanella oneidensis）MR-1的CymA在200 nm大小的脂质体内还原甲基萘醌-7，并且用吸附在SAM修饰的超平金电极上的酶研究了该反应。
It is well established that the structural details of electrodes and their interaction with adsorbed enzyme influences the interfacial electron transfer rate. However, for nanostructured electrodes, it is likely that the structure also impacts on substrate flux near the adsorbed enzymes and thus catalytic activity. Furthermore, for enzymes converting macro-molecular substrates it is possible that the enzyme orientation determines the nature of interactions between the adsorbed enzyme and substrate and therefore catalytic rates. In essence the electrode may impede substrate access to the active site of the enzyme. We have tested these possibilities through studies of the catalytic performance of two enzymes adsorbed on topologically distinct electrode materials. Escherichia coli NrfA, a nitrite reductase, was adsorbed on mesoporous, nanocrystalline SnO2 electrodes. CymA from Shewanella oneidensis MR-1 reduces menaquinone-7 within 200 nm sized liposomes and this reaction was studied with the enzyme adsorbed on SAM modified ultra-flat gold electrodes.
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Menaquinone-7 是四血红素醌醇脱氢酶 CymA 的特异性辅因子。
DOI：
10.1074/jbc.m112.348813
发表时间：
2012-04-20
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
McMillan DG;Marritt SJ;Butt JN;Jeuken LJ
通讯作者：
Jeuken LJ
影响因子：
4.8
作者：
Angove, HC;Cole, JA;Butt, JN
通讯作者：
Butt, JN
电场控制金电极上固定的细胞色素 c 的 pH 依赖性氧化还原过程
影响因子：
3.7
作者：
Jin, Bo;Wang, Gui-Xia;Xia, Xing-Hua
通讯作者：
Xia, Xing-Hua
影响因子：
15
作者：
El Kasmi, A;Wallace, JM;Linderman, RJ
通讯作者：
Linderman, RJ
影响因子：
3.9
作者：
Kemp, Gemma L.;Marritt, Sophie J.;Butt, Julea N.
通讯作者：
Butt, Julea N.