Decreased Type 2 Corticotropin-Releasing Hormone Receptor mRNA Expression in the Ventromedial Hypothalamus during Repeated Immobilization Stress
Decreased Type 2 Corticotropin-Releasing Hormone Receptor mRNA Expression in the Ventromedial Hypothalamus during Repeated Immobilization Stress
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重复固定应激期间下丘脑腹内侧区 2 型促肾上腺皮质激素释放激素受体 mRNA 表达降低
DOI：
10.1159/000054472
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发表时间：
1999
影响因子：
4.1
通讯作者：
Kozo Hashimoto
中科院分区：
文献类型：
--
作者：
S. Makino;K. Asaba;M. Nishiyama;Kozo Hashimoto
慢性或反复应激导致大鼠摄食量减少和体重减轻。应激性厌食症是由于中枢神经系统促肾上腺皮质激素释放激素（CRH）功能增加所致。为了探讨持续应激过程中其他神经肽和外周激素在食物摄入和能量利用中的可能作用，我们检测了重复束缚应激对下丘脑室旁核（PVN）、弓状核（ARC）和下丘脑背内侧核（DMH）等核团CRH、神经肽Y（NPY）、甘丙肽和前阿黑皮素（POMC）mRNA表达的影响，以及血浆胰岛素和瘦素浓度。2型CRH受体（CRHR-2）mRNA在腹内侧下丘脑（VMH），厌食CRH效应的一个可能的目标，也进行了检查。大鼠每天固定2小时，持续6天，并在最后一次固定后24小时处死。固定大鼠的摄食量和体重较低，PVN CRH mRNA水平高于对照组。反复制动还降低血浆胰岛素和瘦素浓度以及VMH CRHR-2 mRNA水平。这些结果为VMH CRHR-2 mRNA水平与血浆瘦素浓度之间的联系提供了额外的证据。ARC NPY和DMH甘丙肽mRNA在反复制动后增加，而ARC POMC mRNA减少。DMH、NPY mRNA和ARC甘丙肽mRNA在制动过程中无明显变化。由于神经肽Y和甘丙肽被认为是促食欲的，而POMC-黑皮质素-4受体系统显然是促食欲的，神经肽mRNA和VMH CRHR-2 mRNA的变化可能对厌食CRH效应起反调节作用。
Chronic or repeated stress results in reduction of food intake and body weight in rats. Stress-induced anorexia has been attributed to increased corticotropin-releasing hormone (CRH) function in the central nervous system. To explore possible roles of other neuropeptides and peripheral hormones involved in food intake and energy utilization during continuing stress, we examined the impact of repeated immobilization stress on expression of mRNAs coding for CRH, neuropeptide Y (NPY), galanin and pro-opiomelanocortin (POMC) mRNAs in such hypothalamic nuclei as the paraventricular nucleus (PVN), arcuate nucleus (ARC) and dorsomedial hypothalamus (DMH), as well as plasma insulin and leptin concentrations. Changes in type 2 CRH receptor (CRHR-2) mRNA in the ventromedial hypothalamus (VMH), a possible target of anorectic CRH effect, were also examined. Rats were immobilized for 2 h daily for 6 days and sacrificed 24 h after the last immobilization. Immobilized rats had lower food intake and body weight and higher levels of PVN CRH mRNA than controls. Repeated immobiliza tion also lowered plasma insulin and leptin concentrations and VMH CRHR-2 mRNA levels. These results provide additional evidence linking VMH CRHR-2 mRNA levels to plasma leptin concentration. ARC NPY and DMH galanin mRNAs increased following repeated immobilization, while ARC POMC mRNA decreased. DMH NPY mRNA and ARC galanin mRNA were unaltered by immobilization. Since NPY and galanin are considered orexigenic, while the POMC-melanocortin-4 receptor system is apparently anorexigenic, the changes in neuropeptide mRNAs and VMH CRHR-2 mRNA may play counterregulatory roles against anorectic CRH effects.
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有证据表明甘丙肽 (GAL)、黑色素浓缩激素 (MCH)、神经降压素 (NT)、阿片黑皮质素原 (POMC) 和神经肽 Y (NPY) 是下丘脑瘦素信号传导的靶标。
DOI：
10.1210/endo.139.2.5909
发表时间：
1998
期刊：
Endocrinology.
影响因子：
--
作者：
Sahu,A
通讯作者：
Sahu,A
幼鼠下丘脑腹内侧核中促肾上腺皮质激素释放因子受体 II 型 (CRF2) 信使核糖核酸水平因母体剥夺而降低。
影响因子：
4.8
作者：
Eghbal-Ahmadi,M;Hatalski,CG;Avishai-Eliner,S;Baram,TZ
通讯作者：
Baram,TZ
影响因子：
15.9
作者：
Schwartz, MW;Seeley, RJ;Baskin, DG
通讯作者：
Baskin, DG
影响因子：
7.7
作者：
Mizuno, TM;Kleopoulos, SP;Mobbs, CV
通讯作者：
Mobbs, CV
下丘脑对饥饿的反应：对消耗性疾病研究的影响。
DOI：
10.1152/ajpregu.1995.269.5.r949
发表时间：
1995
期刊：
The American journal of physiology.
影响因子：
--
作者：
Schwartz,MW;Dallman,MF;Woods,SC
通讯作者：
Woods,SC