Cation Activation of the Basolateral Sodium-Potassium Pump in Turtle Colon
Cation Activation of the Basolateral Sodium-Potassium Pump in Turtle Colon
复制标题
龟结肠基底外侧钠钾泵的阳离子激活
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
D. C. Dawson
中科院分区：
文献类型：
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作者：
D. Halm;D. C. Dawson
甲鱼结肠基侧膜上的电生钠钾交换产生的电流可以直接在用浆膜钡(以阻断基侧钾电导)和粘膜两性霉素B(以降低顶膜的阳离子选择性)处理的组织中测量。我们研究了粘膜钠和血清钾、铷、铯和铵对这一泵电流的激活作用。钠的活化动力学与与泵胞质侧三个独立位置的结合相一致。该泵不被细胞锂离子激活。浆膜阳离子的活化动力学符合与两个独立位置的结合，选择性为Rb&Gt；K&Gt；Cs&Gt；NH4。甲鱼结肠的底侧Na/K泵的性质和动力学至少在性质上与人类红细胞的Na/K-ATPase的性质和动力学相似。
The current generated by electrogenic sodium-potassium exchange at the basolateral membrane of the turtle colon can be measured directly in tissues that have been treated with serosal barium (to block the basolateral potassium conductance) and mucosal amphotericin B (to reduce the cation selectivity of the apical membrane). We studied the activation of this pump current by mucosal sodium and serosal potassium, rubidium, cesium, and ammonium. The kinetics of sodium activation were consistent with binding to three independent sites on the cytoplasmic side of the pump. The pump was not activated by cellular lithium ions. The kinetics of serosal cation activation were consistent with binding to two independent sites with the selectivity Rb > K > Cs > NH4. The properties and kinetics of the basolateral Na/K pump in the turtle colon are at least qualitatively similar to those ofthe well-characterized Na/K-ATPase of the human red blood cell .
影响因子：
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作者：
SCHULTZ, SG
通讯作者：
SCHULTZ, SG