Regulation of toll-like receptors in intestinal epithelial cells by stress and Toxoplasma gondii infection.
Regulation of toll-like receptors in intestinal epithelial cells by stress and Toxoplasma gondii infection.
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DOI：
10.1111/j.1365-3024.2008.01055.x
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发表时间：
2008-11
影响因子：
2.2
通讯作者：
Monroy, F. P.
中科院分区：
文献类型：
--
作者：
Gopal, R.;Birdsell, D.;Monroy, F. P.
肠道上皮细胞(IECS)在入侵的微生物和宿主组织之间形成屏障。IECs表达Toll样受体(TLRs)，识别微生物表面的特定分子信号，激活细胞内信号通路，导致促炎细胞因子和趋化因子的产生。应激激素在促炎细胞因子的调节和免疫反应的下调中起重要作用。结果表明，感染弓形虫后第8天，TLR-2、TLR-4、TLR-9和TLR-11在小鼠IECs中的表达水平显著升高。相比之下，冷水应激(CWS+INF)感染小鼠的TLRs表达显著降低。小鼠MODE-K细胞株感染后TLR-9和TLR-11的表达显著增加。与仅暴露于寄生虫的细胞相比，暴露于去甲肾上腺素(NE)和寄生虫的细胞中TLR-9和TLR-11的表达显著降低。与INF组相比，CWS+INF组TLRs的负性调节因子SIGIRR显著增加。应激成分能够降低IECs中TLRs的表达水平，降低寄生虫负荷，增加负性调节因子的表达，从而改善C57BL/6小鼠口服感染弓形虫时常见的肠道炎症反应。
Intestinal epithelial cells (IECs) form a barrier between invading microorganisms and the underlying host tissues. IECs express Toll-like receptors (TLRs) that recognize specific molecular signatures on microbes which activate intracellular signaling pathways leading to production of proinflammatory cytokines and chemokines. Stress hormones play an important role in modulation of proinflammatory cytokines and downregulation of immune responses. Here we demonstrated that expression levels of TLR-2, TLR-4, TLR-9 and TLR-11 were significantly increased in mouse IECs following infection with Toxoplasma gondii on day 8 post infection. In contrast, expression of TLRs was significantly decreased in infected mice subjected to cold water stress (CWS+INF). Expression of TLR-9 and TLR-11 in the mouse MODE-K cell line was significantly increased after infection. Expression of TLR-9 and TLR-11 in cells exposed to norepinephrine (NE) and parasites was significantly decreased when compared to cells exposed to parasites only. A significant increase was observed in SIGIRR, a negative regulator of TLRs in the CWS+INF group when compared to the INF group. Stress components were able to decrease expression levels of TLRs in IECs, decrease parasite load, and increase expression of a negative regulator thereby ameliorating intestinal inflammatory responses commonly observed during per oral T. gondii infection in C57BL/6 mice.
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影响因子：
6
作者：
Cario, E;Brown, D;Podolsky, DK
通讯作者：
Podolsky, DK
影响因子：
15.9
作者：
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通讯作者：
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通讯作者：
Evengård, B